石氏“醒脑开窍针法”对脑缺血再灌模型大鼠血浆AngⅡ、CGRP及ET含量的影响  

Influences of Shi's acupuncture therapy of awakening brain and opening orifices on plasma content of AngII,CGRP and ET in rat model of cerebral ischemia-reperfusion

作　　者：郑宇 张露芬[2] 任晓暄[2] 王春生 张帆 闫明茹[5] 周登芳[6] 郑玲[7] 孙洁[2] 魏惠芳[2] 

机构地区：[1]中国核工业北京401医院,北京102413 [2]北京中医药大学针灸推拿学院 [3]北京回龙观社区卫生服务中心 [4]北京市中医医院 [5]中国中医科学院广安门医院 [6]北京市肿瘤医院 [7]北京医院

出　　处：《北京中医药大学学报》2011年第2期140-144,共5页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的观察石氏"醒脑开窍针法"对血管紧张素Ⅱ(AngⅡ)、降钙素基因相关肽(CGRP)及内皮素(ET)在脑缺血再灌模型大鼠中的作用和意义。方法线栓法制备脑缺血再灌大鼠模型,采用不同介入时间用石氏"醒脑开窍"针法和香丹注射液给予治疗,于模型制备成功后72 h取材,采用放射免疫分析方法检测血浆中AngⅡ、CGRP及ET含量。结果针刺可不同程度逆转由脑缺血再灌所致模型大鼠血浆中CGRP与AngⅡ、ET的比值失调。结论针刺早期介入治疗对肾素AngⅡ系统、ET和CGRP的调节在脑缺血再灌的发生发展中起重要作用,石氏"醒脑开窍针法"能从整体性调节入手,促使体内血浆AngⅡ、CGRP、ET的失调含量趋于正常水平,具有治疗的有效性和疗程治疗的必要性。Objective To observe the effects and significance of Shi＇s acupuncture therapy of awakening brain and opening orifices on the plasma content of angiotensin Ⅱ（AngII）,calcitonia gene related peptide（CGRP） and endothelin（ET） in rat model of cerebral ischemia-reperfusion.Methods The rat model was established by using thread approach,and then treated with Shi＇s acupuncture therapy of awakening brain and opening orifices and Xiangdan Injection at different intervention times.After successful modeling for 72 hours,the plasma content of AngII,CGRP and ET were detected by applying radioimmunoassay.Results Acupuncture reverted the disordered ratio between CGRP and AngII or ET in rat model of cerebral ischemia-reperfusion in varying degrees.Conclusion The early intervention of acupuncture plays an important role in the development of cerebral ischemia-reperfusion through regulating rennin-angiotensin system,ET and CGRP.Shi＇s acupuncture therapy of awakening brain and opening orifices can correct the abnormal content of AngII,CGRP and ET based on holistic regulation,which has therapeutic effectiveness and necessity of treatment course.

关 键 词：醒脑开窍针法 脑缺血再灌注 血管紧张素Ⅱ 内皮素 降钙素基因相关肽 大鼠 

分 类 号：R245.3[医药卫生—针灸推拿学]