利用基于扫描相机的荧光寿命成像显微技术研究细胞周期  

Study on Cell Cycle Using Fluorescence Lifetime Imaging Microscopic System Based on a Streak Camera

作　　者：王岩[1] 赵羚伶[2] 陈同生[3] 许改霞[1] Artem Pliss Tymish Y.Ohulchanskyy Paras N.Prasad 屈军乐[1] 牛憨笨[1] 

机构地区：[1]深圳大学光电子学研究所光电子器件与系统教育部重点实验室,广东深圳518060 [2]纽约州立大学布法罗分校激光、光子学和生物光子学研究所,纽约布法罗14260-3000 [3]华南师范大学激光生命科学教育部重点实验室及激光生命科学研究所,广东广州510631

出　　处：《中国激光》2011年第3期127-132,共6页Chinese Journal of Lasers

基　　金：国家自然科学基金(60627003,61078067)资助课题

摘　　要：利用基于扫描相机的荧光寿命成像显微系统,以细胞周期为模型,研究转染绿色荧光蛋白的HeLa细胞的荧光寿命。结果表明,处于周期内不同进程的细胞的荧光寿命为2.50-3.00 ns。处于分裂期的细胞的荧光寿命在1 h内从2.86 ns下降到2.82 ns;在DNA合成前期的8 h内,荧光寿命从2.82 ns下降到2.78 ns。荧光寿命的差异反映了细胞周期中核浆内大分子浓度的变化,对了解细胞周期的分子机制有一定的意义。The fluorescence lifetime of the HeLa cells which are transfected with green fluorescent proteins during the cell cycle is investigated using fluorescence lifetime imaging microscopic system based on a streak camera.Experimental results show that fluorescence lifetime of HeLa cells is between 2.50 ns and 3.00 ns during different processes of cell cycle.The fluorescence lifetime of the mitosis cell drops to 2.82 ns from 2.86 ns within one hour,and it further drops to 2.78 ns from 2.82 ns during the roughly eight hours of the pre-DNA-synthetic phase（G1-phase）.The difference of the fluorescence lifetime implies that the macromolecular concentration in the nucleoplasm of the cell nucleus changes throughout the cell cycle,the study of which is significant to the understanding of macromolecular processes,kinetics and concentrations in the nucleoplasm of the cell nucleus throughout the cell cycle,as well as the regulation of cell cycles.

关 键 词：成像系统 扫描相机 荧光寿命成像 细胞周期 HELA细胞 

分 类 号：TB872[一般工业技术—摄影技术] Q334[生物学—遗传学]