浅谈定心胶囊中丹参酚酸B的含量测定  

Measurement of Salvianolic-acid B in Dingxin Capsule

作　　者：王丹[1] 张艳[1] 张军[2] 刘晓蕾[3] 

机构地区：[1]辽宁中医药大学附属医院,沈阳110032 [2]辽宁中医药大学 [3]沈阳市急救中心

出　　处：《中国医疗前沿》2011年第2期79-80,共2页China Healthcare Innovation

摘　　要：目的以高效液相色谱法测定定心胶囊中有效成分之一丹参酚酸B的含量,以明确其作用机理。方法采用Hypersil ODS(5μ,4.6mm×200mm)色谱柱,甲醇-乙腈-甲酸-水(30:10:1:59)为流动相,检测波长286nm。结果丹参酚酸B在0.1024μg～0.6146μg范围内呈良好的线性关系,r=0.9999;平均回收率为96.66%(n=9),RSD=0.9%。结论所建立的方法稳定、可靠,可为该产品建立质量控制方法提供参考。Objective To explore the mechanism of Dingxin Capsule by determining one of the effective ingredients（Salvianolic acid B）.Methods Chromatography was set of Hypersil ODS（5μ,4.6mm×200mm）,mobile phase：MeOH-CAN-Hac-H2O（3010159,V/V）,with gradient elution,wavelength tested was 286nm.Results The methodological study showed that a good linear correlation existed in the range of 0.1024-0.6146μg of the Salvianolic-acid B applied,r=0.9999.The average recovery was 96.66%（n=9） with the RSD of 0.9%.Conclusion The method is simple,reproducible and accurate to the quality control for Funing Suppository.

关 键 词：定心胶囊 高效液相色谱法 丹参酚酸B 

分 类 号：R927.2[医药卫生—药学]