玉米细菌干茎腐病菌成团泛菌的种子传播  

Seed transmission of Pantoea agglomerans,causal agent of dry stalk rot,in maize

作　　者：曹慧英[1] 李洪杰[1] 朱振东[1] 王晓鸣[1] 

机构地区：[1]中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《植物保护学报》2011年第1期31-36,共6页Journal of Plant Protection

基　　金：农业部作物种质资源保护项目(NB08-2130135-(25-30)-21);国家科技支撑计划(2006BAD08A06)

摘　　要：为明确成团泛菌Pantoea agglomerans引起的玉米细菌干茎腐病经种子传播的规律,采用细菌常规分离法、Sherlock微生物鉴定系统、特异性分子检测技术,对与干茎腐病相关的杂交种金玉9856及其父本PS056、母本OSL190进行了种子带菌检测,证明金玉9856和PS056种子内部带菌,获得分离物Pag1和Pag2,2个分离物对感病的PS056均具有致病性。菌液浸种、种子注射接种和自然带菌种子直播都能够引发干茎腐病,发病率分别达到100%、100%和80%,而持续高温(50℃)处理4天的种子则在植株上不表现症状。对种子接种后长成的植株的系统检测证明,成团泛菌侵染种子后,通过植株维管束系统向地上部组织扩展,随着水分的运输,病菌通过茎秆到达果穗的籽粒中,完成从种子到植株、再到新种子的病害循环,同时能够引起植株发病。Bacterial dry stalk rot caused by Pantoea agglomerans, is a new disease in maize seed produc- tion field. For understanding the transmission of P. agglomerans in maize seed, the detection of seeds of inbred line Jinyu 9856, male parent line PS056 and female parent OSL190 were conducted using three methods including normal method, Sherlock Microbial Identification System and molecular species-specif- ic detection method. P. agglomerans was detected in and isolated from seeds of PS056 and Jinyu 9856, but not in OSL190. Two isolates were pathogenic and causing dry stalk rot in plants of PS056. P. agglo- merans, inoculated onto seeds of PS056 by soaking, injecting and nature infection, can cause typical symptoms of dry stalk rot, and the infection percentage was 100%, 100% and 80%, using three inocu- lation methods respectively, and there were not symptoms by seed treatment with high temperature （50 ℃ ） for four days. The result of systematic detection showed that P. agglomerans, inoculated on seeds, can move up to maize plant and it can reach to leaves and seeds through stem along vascular system. All results mean that P. agglomerans successfully cycled from seed to new seed.

关 键 词：玉米 细菌干茎腐病 成团泛菌 种子带菌 病害循环 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]