PCR-DGGE研究臭氧耦合ASBR/SBR控氮磷污泥减量化工艺中的细菌多样性  

Bacterial Diversity of the Combined Ozonation-ASBR/SBR Sludge Reduction with Simultaneous Nitrogen and Phosphorus Removal Process Using PCR-DGGE

作　　者：赵林林[1,2] 王海燕[1] 杨慧芬[2] 何赞[2] 周岳溪[1] 张乐[3] 庞朝辉[3] 

机构地区：[1]中国环境科学研究院水污染控制技术研究室,北京100012 [2]北京科技大学土木与环境工程学院,北京100083 [3]华北水利水电学院土木与交通学院,河南郑州450011

出　　处：《环境工程技术学报》2011年第2期123-130,共8页Journal of Environmental Engineering Technology

基　　金：国家"十一五"科技支撑计划项目(2009BADC2B01-02);中国环境科学研究院中央级公益性科研院所基本科研业务专项项目(2007KYYW10)

摘　　要：采用分子生物学手段PCR-DGGE技术对臭氧耦合ASBR/SBR控氮磷污泥减量化工艺中的ASBR和污泥减量化前后的SBR中的细菌多样性进行了研究。结果表明,ASBR中的主要细菌类群为Firmicutes类群,β-pro-teobacterium类群,γ-proteobacterium类群和Bacteroidetes类群;污泥减量化后SBR中细菌丰富度与减量前相比有所增加,但是相似性指数极高,为85%;臭氧施加进行污泥减量对SBR中的优势菌群多样性影响不大,污泥减量化前后SBR反应器内均存在Firmicutes bacterium,Candidate division TM7,ammonia-oxidizingbacterium,Bacteroidetes bacterium,β-proteobacterium,Denitrifying bacterium,Pseudomonassp和Nitrosococcus halophilus Nc4,其中减量化后Bacteroidetesbacterium,β-proteobacterium,Denitrifying bacterium和Nitrosococcus halophilus Nc4的数量略高于污泥减量化之前。The bacterial diversity in the ASBR and SBR of the combined process,i.e.combined ozonation-ASBR/SBR sludge reduction with simultaneous nitrogen and phosphorus removal process was studied by molecular biological PCR-DGGE method before and after sludge reduction.The results indicated that the dominant bacterial communities in the ASBR were Firmicutes bacterium,β-proteobacterium,γ-proteobacterium and Bacteroidetes bacterium.The bacterial richness in SBR after sludge reduction was slightly increased compared with that before sludge reduction,but with high similarity（85%）.The dominant bacteria diversity of SBR was not influenced significantly by the ozone dosage for sludge reduction.There were Firmicutes bacterium,Candidate division TM7,ammonia-oxidizing bacterium,Bacteroidetes bacterium,β-proteobacterium,Denitrifying bacterium,Pseudomonas spand Nitrosococcus halophilus Nc4 in SBR both before and after sludge reduction.However,the number of Bacteroidetes bacterium,β-proteobacterium,Denitrifying bacterium and Nitrosococcus halophilus Nc4 in SBR after sludge reduction was slightly higher than that before sludge reduction.

关 键 词：ASBR/SBR 臭氧耦合 污泥减量化 PCR-DGGE 

分 类 号：X703[环境科学与工程—环境工程]