一氧化氮在一次高负荷运动诱导大鼠腓肠肌线粒体生物合成的转录调控作用  

NO Effects of Transcriptional the Regulation of Rat Gastrocnemius Mitochondria Biosynthesis after a High-load Exercise

作　　者：陈英杰[1] 赵广高[2] 

机构地区：[1]阜阳师范学院体育学院,安徽阜阳236037 [2]南昌大学体育系,江西南昌330031

出　　处：《体育科学》2011年第3期58-62,67,共6页China Sport Science

摘　　要：目的:观察一次高负荷运动后NO介导腓肠肌线粒体生物合成的转录调控作用。方法:6周龄雄性SD大鼠56只,随机分成:安静对照组(C)、运动后即刻组(E0)、运动后3 h组(E3)、6 h组(E6)、12 h组(E12)、18 h组(E18)和24 h组(E24);硝酸还原酶法测腓肠肌NO浓度和NOS活性,放射性免疫法测cGMP含量;RT-PCR测eNOS、PGC-1α、NRF-1、Tfam和COXIV基因;Western-blotting测COXIV蛋白。结果:E0组腓肠肌NO浓度、cGMP含量升高,NOS活性增加,eNOS mRNA下降;E6组NOS活性、eNOS mRNA和cGMP含量都达到峰值。E0组腓肠肌NRF-1 mRNA、Tfam mRNA、PGC-1α和COXIV mRNA升高。结论:一次高负荷运动可能通过NO-NOS-cGMP通路激活、调控NO体系,进而激活sGC-cGMP依赖信号途径,激发线粒体生物合成。Objective：To observe the variation of gastrocnemius muscle NO-NOS-Cgmp pathway and NO effects the gastrocnemius muscle transcriptional regulation of mitochondrial biogenesis after a high-load exercise.Methods：56 male SD rats（6-week-old） randomly divided into quiet control group（C）,0-hour after treadmill exercise group（E0）,3 h（E3）,6 h（E6）,12 h（E12）,18 h（E18） and 24 h（E24） group.Determinate gastrocnemius muscle NO concentration and NOS activity through the method of nitrate reductase,determinate gastrocnemius muscle cGMP content through the method of radioimmunoassay;determinate gastrocnemius muscle eNOS mRNA,PGC-1α mRNA,NRF-1 Mrna,Tfam mRNA and COXIV mRNA expression through the method of RT-PCR;determinate gastrocnemius muscle COXIV protein content by Western-Blotting method.Results：E0 group gastrocnemius muscle NO concentration,cGMP content and NOS activity was increased,eNOS mRNA was decreased,E6 group NOS activity,eNOS mRNA and cGMP content all reached the peak.E0 group gastrocnemius muscle NRF-1mRNA,Tfam mRNA,PGC-1αmRNA and COXIVmRNA was increased.Conclusion：A high-load exercise can regulate gastrocnemius muscle NO system with its NO-NOS-cGMP pathway,and NO activate sGC-cGMP signaling pathway,thus inspire mitochondrial biogenesis.

关 键 词：高负荷运动 腓肠肌 一氧化氮 环一磷酸鸟苷 线粒体 生物合成 鼠 动物实验 

分 类 号：G804.7[文化科学—运动人体科学]