猪ATGL基因5′调控区的SNPs检测及生物信息学分析  

SNPs Detection and Bioinformatics Analysis on 5′ Regulatory Region of the Porcine ATGL Gene

作　　者：华绪川[1,2] 张立凡[1] 蒋晓玲[1] 翟继鹏[1] 徐宁迎[1] 张金枝[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310029 [2]浙江省畜牧兽医局,浙江杭州310020

出　　处：《中国畜牧杂志》2011年第5期5-9,共5页Chinese Journal of Animal Science

基　　金：国家重点基础发展规划项目(2006CB102100);浙江省科技厅重大项目(2005C122005-2);浙江省博士后科研择优资助项目(2008X80801)

摘　　要：脂肪甘油三酯水解酶(ATGL)是脂肪组织脂肪动员过程中的水解限速酶,主要催化甘油三酯水解为甘油二酯。研究对金华猪、岔路黑猪、杜洛克、大约克和皮特兰5个猪种ATGL基因其5′调控区1.2 kb的片段进行SNPs检测和生物信息学分析。结果表明:ATGI基因5'调控区存在第-845位G→C和第-854位T→C的连锁突变。序列分析显示该区域可能存在启动子区,且2个突变都会导致其部分潜在转录因子结合位点的产生或消失。采用PCR-RFLP方法检测g-845G→C座位在金华猪、岔路黑猪、杜洛克、大约克和皮特兰中的分布情况,卡方分析结果显示,3种基因型在5个猪种中的分布存在极显著差异(P<0.01),提示不同猪种间脂肪性状的差异可能与ATGL基因5'调控区的基因突变有关。As a key enzyme in the initial step of triglyceride hydrolysis, adipose triglyceride lipase （ATGL） plays a critical role in the lipolytic catabolism of stored fat in adipose tissue. 1.2 kb of the 5＇ flanking region of the porcine A TGL gene was sequenced in this study and two completely linked mutations, g-845G→C and g-854T→C, were found in the region. Results of the bioinformatics analysis indicated the presence of promoter sequence and mutations in loci g- 845G→C and g-854T→C could create or destroy potential transcription factor binding sites. Locus g-845G→C were genotyped in Jinhua, Chalu black, Large Yorkshire, Duroc and Pietrain pig breeds by PCR-RFLP, and the results showed that the distribution of three genotypes was significantly different among breeds （P〈0.01）, which suggested that the g- 845G→C mutation may eontribute to diversity of fat traits in different pig breeds.

关 键 词：ATGL基因 脂肪性状 序列分析 PCR—RFLP 猪 

分 类 号：S828.2[农业科学—畜牧学]