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作 者:陈志敏[1,2] 陈冠华[1] 郝庆红[1] 王桢[1] 郭云霞[1]
机构地区:[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学食品科技学院,河北保定071001
出 处:《河北大学学报(自然科学版)》2011年第1期57-62,共6页Journal of Hebei University(Natural Science Edition)
基 金:河北省自然科学基金资助项目(202281)
摘 要:在适当的条件下,强力霉素可与镁离子形成具有强荧光的络合物,在溴化十六烷基三甲铵胶束体系中,荧光可进一步增强.据此建立了一种测定强力霉素残留的新方法.在优化条件下,强力霉素线性范围为0.2~10mg/L,相对标准偏差为0.9%~3.9%,检出限为12μg/kg,加标回收率90.5%~107.3%.该方法已应用于猪肉强力霉素残留的测定.Doxycycline can form hyperfluorescence compound with Mg2+ under proper conditions.The fluorescence of the compound can be futher increased in cetyltrimethyl-ammonium bromide micellar.A new assay was developed to determine the doxycycline residue based on this principle.Under the optimum conditions,the linear dynamic range was 0.2-10 mg/L;the relative standard deviation was between 0.9% and 3.9%;the detection limit was 12 μg/kg;the recoveries of spiked sample were between 90.5% and 107.3%.The method has been applied to the determination of doxycycline residue in pork.
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