香蕉炭疽菌拮抗生防菌株的筛选及鉴定  

Screening and Identification of Antagonistic Organisms against Banana Anthracnose Fungus

作　　者：罗萍[1] 区建发[1] 杨炜钊[1] 方祥[1] 

机构地区：[1]华南农业大学食品学院生物工程系,广东广州510642

出　　处：《现代食品科技》2011年第3期275-278,共4页Modern Food Science and Technology

摘　　要：从香蕉组织中分离筛选到18株在平板上对香蕉炭疽菌孢子有明显抑菌活性的菌株,将这18株菌分别与香蕉炭疽菌共同接种到香蕉果指上进行活体防效复筛,发现与清水对照组相比较,AcT-02、AcT-07、AcT-09、AcT-11、AcT-13共5株细菌均表现出显著的防治效果,防效分别为49.77%±4.20%、47.87%±8.97%、63.82%±3.16%、68.15%±2.48%和73.85%±2.39%,香蕉病情指数显著降低;将该5株菌进行香蕉自然发病的防效试验,发现只有AcT-13菌株具有显著防治效果,其病情指数为18.67%,比对照降低了13%,将AcT-13进行形态学观察、生理生化特性测定以及分子学序列分析,初步将AcT-13菌株鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens。18 Strains of antagonistic were isolated from plant tissue of banana,which showed strong square inhibition zone in plate containing banana anthracnose spore suspension.And then antagonistic experiments in vivo of banana by artificial infection with banana anthracnose and antagonistic strains were carried out.the result showed that,compared to the control of water,only AcT-02,AcT-07,AcT-09,AcT-11,and AcT-13 could decrease banana anthracnose,with their control effect being of 49.77%±4.20%,47.87%±8.97%,63.82%±3.16%,68.15%±2.48% and 73.85%± 2.39%,respectively.The disease index significantly discreased.The antagonistic experiment by natural infection with banana anthracnose using the five antagonistic strains showed that only the AcT-13 fermentation can decreased the disease index of banana anthracnose and the disease index of banana anthracnose was 18.67%,being 13% lower than that of the control.Trough morphological observation,physiological and biochemical characterristics,and analysis of gene sequense,the AcT-13 was identified as Bacillus amyloliquefaciens.

关 键 词：香蕉炭疽菌 拮抗菌 生物防治 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]