恒古骨伤愈合剂对兔激素性股骨头坏死模型超微结构的影响  

Ultrastructural evaluation of the SANFH rabbit animal models intervened by Osteoking

作　　者：胡敏[1] 赵宏斌[2] 钱传云[2] 魏蔚[2] 徐宏慧[2] 唐薇[2] 罗德军[2] 张扬[2] 

机构地区：[1]昆明学院医学院,昆明650214 [2]昆明医学院第一附属医院,昆明650032

出　　处：《中华中医药杂志》2011年第3期486-489,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81060361;No.30860389);云南省自然科学基金资助项目(No.2010ZC169);云南省教育厅科学研究基金项目(No.2010C212);昆明市科技计划重点项目(No.10S090202)~~

摘　　要：目的:观察恒古骨伤愈合剂干预激素性股骨头坏死兔模型的电子显微镜观察改变。方法:将150只新西兰白兔按随机数字表法分为空白对照组(A组)24只,正常兔服用恒古骨伤愈合剂对照组(B组)24只,实验组102只。对实验组耳缘静脉注射大肠杆菌内毒素(10μg/kg)2次,注射间隔24h。并于第2次注射大肠杆菌内毒素后,臀肌注射甲基强的松龙粉针剂(20mg/kg)3次,注射间隔24h,制备兔激素性股骨头缺血坏死模型。于造模后第5周,将造模成功的53只新西兰白兔以随机数字表法分配其中的48只至模型组(C组,造模后不喂恒古骨伤愈合剂)和恒古骨伤愈合剂治疗组(D组,造模后喂恒古骨伤愈合剂),每组24只。B组和D组以恒古骨伤愈合剂(按经由人与兔体表面积用药量换算计算出的剂量)灌胃,1次/2d;A组和C组以等体积的蒸馏水灌胃。造模后8、12、16周,截取股骨头行透射电镜和扫描电镜观察。结果:透射电镜和扫描电镜观察显示A、B两组股骨头骨细胞及骨基质正常,细胞核呈卵圆形位于细胞一端,核膜完整,细胞质内线粒体丰富;C组可见骨细胞变性坏死,骨髓腔出血,骨髓腔毛细血管狭窄,血管内皮细胞受损;D组骨细胞变性、骨髓腔出血和狭窄程度均较轻,可见较多的成骨细胞、破骨细胞、新生骨细胞及骨基质,新生骨形成小梁。结论:恒古骨伤愈合剂可有效促进早期激素性股骨头坏死兔模型坏死股骨头的修复。Objective： To evaluate the ultrastructural changes on the femoral heads of the SANFH rabbit animal models and after it were intervened by Osteoking（herbs of the Yi minority in Yunnan province） using general and light microscope observation.Methods： A total of 150 New Zealand white rabbits were randomly divided into a non-treatment control group（A group,n=24）,normal rabbits with Osteoking treatment group（B group,n=24）,and the experimental group（n=102）.The experimental group was injected with escherichia coli endotoxin（10μg/kg） into auricular vein twice by 24-hour intervals,and prednisolone（20mg/kg） was injected into buttock three times by 24-hour intervals to make steroid-induced femoral head necrosis model.At the fifth week,48 out of 53 rabbits were equally divided into model group（C group,n=24,models with non-treatment Osteoking） and abnormal rabbits with Osteoking treatment group（D group,n=24）.B group and D group were intragastrically administrated with Osteoking,once every two days.A group and C group were intragastrically administrated with the equal volume of saline.At 8th,12th and 16th week after model preparation,the femoral head specimens were observed under the electron microscopy.Results： Observed with transmission electron microscope and scanning electron microscope,it showed that the bone cells and bone matrix of femoral heads A and B groups were normal.Its oval nucleus located at one end of cells,the nuclear membrane was integrity and its cytoplasm was rich in mitochondria.But,the C group was observed the degeneration and necrosis of bone cells,the hemorrhage and the narrow capillaries of bone marrow cavity,and the damage of the vascular endothelial cell.Observesion with the D group showed that the same appearance as the group C,but the less necrosis in the D group.And it was observed that osteoblasts,osteoclasts,new bone cells,bone matrix and neoplastic bone formation were more than those in C group.Conclusion： It suggested that Osteoking could effectively he

关 键 词：恒古骨伤愈合剂 激素性股骨头坏死 骨组织 电子显微镜 

分 类 号：R285.5[医药卫生—中药学]