HPLC法测定枳壳不同炮制品中柚皮苷、橙皮苷和新橙皮苷的含量  

Contents determination of Naringin,hesperidin and neohesperidin in processed products of Fructus Aurantii by HPLC

作　　者：蒋以号[1] 吕妍[2] 曹旻旻[3] 张庆华[4] 龚千锋[3] 

机构地区：[1]南昌大学,南昌330031 [2]南昌大学第四附属医院,南昌330003 [3]江西中医学院,南昌330004 [4]广西中医学院,南宁530001

出　　处：《中华中医药杂志》2011年第3期601-603,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江西省科技厅重大项目(No.赣科发计字[2007]189号)~~

摘　　要：目的:用HPLC法对枳壳的江西炮制方法及其药典法炮制品中柚皮苷、橙皮苷和新橙皮苷含量进行测定。方法:色谱柱:E1823028 C18(250mm×4.6mm,5μm);流动相为乙腈-0.1%磷酸水(23:77),检测波长UV 285nm,流速1.0mL/min,柱温30℃。结果:柚皮苷在0.596-5.960μg范围内线性关系良好(r=0.9997),橙皮苷在0.106-1.060μg范围内线性关系良好(r=0.9996),新橙皮苷在0.470-4.700μg范围内线性关系良好(r=0.9999)。柚皮苷的平均回收率为101.17%(RSD=1.26%),新橙皮苷的平均回收率为101.28%(RSD=1.34%),橙皮苷的平均回收率为101.20%(RSD=0.895%)。结论:该方法简单、准确,可用于枳壳药材的质量控制;樟帮炮制法对枳壳的成分影响较大,并且有新的成分产生。Objective： To determine the contents of Naringin,hesperidin and neohesperidin in Fructus Aurantii and its products processed by Jiangxi methods and pharmacopoeia metheds by HPLC.Methods： E1823028 C18 Column（250mm×4.6mm,5μm）,mobile phase of acetonitrile-0.1%phosphoric acid（volume ratio of 23：77）,detection wavelength at 285nm.flow phase rate being 1.0mL/min and the column temperature at 30℃.Results： The calibration curves were linear in the range of 0.596-5.96μg for narngin（r=0.9997）,0.106-1.06μg for hesperidin（r=0.9996）and 0.470-4.70μg for neohesperidin（r=0.9999）.The average recovery of naringin was 101.17%,（RSD=1.26%）,the average recovery of hesperindin was 101.2%（RSD=0.895%） and that of neohesperindin was 101.28%（RSD=1.34%）.Conclusion： This method is simple,accurate and reliable for the quality control of Fructus Aurantii.

关 键 词：枳壳 樟帮饮片 柚皮苷 橙皮苷 新橙皮苷 含量测定 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]