不同种质黄芩蛋白质电泳的鉴别研究  

Identif ication study on protein gel electrophoresis of different germplasm of Baicalin

作　　者：林慧彬[1] 路俊仙[1] 崔璐[2] 韩丽丽[2] 李真[2] 林建群[3] 林建强[3] 

机构地区：[1]山东省中医药研究院中药资源研究室,济南250014 [2]山东中医药大学,济南250014 [3]山东大学生命科学院微生物技术国家重点实验室,济南250100

出　　处：《中华中医药杂志》2011年第3期606-608,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2009年山东省农业良种工程重大课题(No.2009LZ201-03);2007年山东省中医药发展计划课题(No.2007-120)~~

摘　　要：目的:研究比较不同种质黄芩蛋白质的差异。方法:采用蛋白质聚丙烯酰胺凝胶电泳方法。结果:沙滩黄芩的蛋白质主要分布在相对分子量10.0-70.0kDa之间,且分布较均匀,一级条带主要集中在20.0-30.0kDa;不同产地黄芩的蛋白质条带在相对分子量66.0、70.0、112.0kDa处染色较深,蛋白质含量较高,与沙滩黄芩有显著差异。花粉红色及玫红色的黄芩,电泳条带数目较少,染色较浅,但其一级条带和紫花黄芩的条带基本相同。结论:黄芩与沙滩黄芩的蛋白质电泳差别大于不同种质黄芩之间的差异。证实蛋白质电泳结果较相近的黄芩具有较近的亲缘关系。Objective： To compare the protein difference of different germplasm of Baicalin.Methods： The method of protein gel electrophoresis was used.Results： The protein of S.strigillosa was mainly between 10.0~70.0 kDa and its first-order stripe distributed over 20.0-30.0 kDa.The electrophoresis spectrum was shown that different germplasm of Baicalin had deeper color stripe at 66.0,70.0,112.0 kDa and had more difference with S.strigillosa.Conclusion： The difference of protein gel electrophoresis was larger between Baicalin and S.strigillosa than that of different germplasms of Baicalin.It was verified that Baicalins which had similar results of protein gel electrophoresis had closer relationship.

关 键 词：黄芩 种质 蛋白质电泳 

分 类 号：R284.1[医药卫生—中药学]