柳蘑生物学特性及ITS序列分析  

Biological Characteristics and ITS Sequence Analysis of Pholiota adiposa

作　　者：张薇[1] 谢凤颖[1] 王占斌[1] 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《黑龙江农业科学》2011年第3期18-20,共3页Heilongjiang Agricultural Sciences

基　　金：中央高校基本科研业务费专项资金资助项目(DL09EAQ02);东北林业大学本科生基金资助项目

摘　　要：对柳蘑生物学特性进行比较研究,结果表明：柳蘑菌丝生长适宜温度为20-30℃,最适温度为25℃;生长的最适光照条件是全黑暗;生长的pH为5-11,最适生长pH为6;最适碳源为蔗糖,葡萄糖次之;最适氮源为硝酸钾,谷氨酸次之。核糖体转录间隔区（Internal Transcribed Spacer）PCR扩增结果表明,其属担子菌与已知大型真菌Pholiota adiposa序列同源性达99%。The biological characteristics of Pholiota adiposa were compared.The results showed that the temperatures appropriate to its mycelia growth was 20～30℃,the best one was 25℃.The best illumination condition was entire darkness.The pH appropriate to its mycelia growth was 5～9,the best one was 6.The best carbon source was sucrose,the second was glucose.The internal transcribed spacer region（ITS）was sequenced to determine whether the DNA sequence data supported the experimental result.The phylogenetic tree for the 19 pieces of homologous sequences were analyzed with the highest homology reaching 99%.

关 键 词：柳蘑 生物学特性 核糖体转录间隔区 

分 类 号：S646.19[农业科学—蔬菜学]