柑橘类植物和其它植物的GPAT和SOD基因分子进化分析  

Molecular Evolution Analysis of GPAT and SOD Genes in Citrus and Other Plants

作　　者：吴波[1] 刘勇[2] 

机构地区：[1]江西中医学院/江西省中药种质资源工程技术研究中心,江西南昌330004 [2]江西农业大学农学院,江西南昌330045

出　　处：《江西农业大学学报》2011年第1期162-167,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省自然科学基金项目(2009GZN0024);江西省教育厅科技项目(GJJ09542);江西农业大学博士启动基金项目(1791)

摘　　要：甘油-3-磷酸酰基转移酶(GPAT)基因和超氧化物歧化酶(SOD)基因是与植物抗寒和抗逆密切相关的2个基因。为了研究柑橘类植物之间以及和其它植物之间上述两基因的分子进化的异同,已经获得的数种柑橘类植物的GPAT基因、SOD基因氨基酸序列和通过检索GenBank数据库获得的相关序列为试验数据,使用PAUP4.0软件,通过最大简约法,分别构建GPAT基因、Fe/Mn-SOD基因、Cu/Zn-SOD基因和总SOD基因的分子系统进化树。GPAT基因分子系统进化树分析结果表明,GPAT基因在单子叶和双子叶植物中的进化差异不大,在柑橘类植物中的进化差异较大;Fe/Mn-SOD基因分子系统进化树分析结果表明,该基因分子进化与植物自身的抗逆能力有密切关系;Cu/Zn-SOD基因分子系统进化树分析结果表明,该基因在进化上是相当保守的。总SOD基因分子系统进化树分析结果表明,SOD基因的进化和聚类并不是以植物的科属是否相同或相近为依据的,而是以该基因所结合的金属离子的不同来分类的;Cu/Zn-SOD基因和Fe/Mn-SOD基因来源于不同的祖先基因,前者较后者保守。GPAT and SOD are very important genes closely related with cold resistance and stress resistance of plants.In order to study the similarities and differences of GPAT and SOD genes,molecular evolution,the systemic evolution trees of GAPT gene and Fe/Mn-SOD and Cu/Zn-SOD and total SOD gene were established by using the software of PAUP4.0 and the method of MP in GPAT and SOD genes,amino acids sequences of several citrus and other plants in database of GenBank.The analysis of GPAT gene＇s systemic evolution tree showed that the molecular evolution difference in monocotyledons and dicotyledons was not so large but the difference was relatively large in citrus plants.The analysis of Fe/Mn-SOD gene＇s systemic evolution tree showed that this gene＇s molecular evolution had close connection with stress resistance of the plant itself.The analysis of Cu/Zn-SOD gene＇s systemic evolution tree showed that the gene＇s evolution was very conservative.The analysis of total SOD gene＇s systemic evolution tree showed that the foundation of the evolution and clustering in SOD gene was not plants,family and genus but the metallicions of SOD gene.Cu/Zn-SOD gene and Fe/Mn-SOD gene rooted in distinct ancestral gene and the former was more conservative than the latter.

关 键 词：柑橘类植物 GPAT SOD 分子进化分析 

分 类 号：Q943.2[生物学—植物学]