转基因小鼠用作脊髓灰质炎活疫苗神经毒力实验动物模型的研究  

TRANSGENIC MICE AS A MODEL FOR NEUROVIRULENCE TEST OF LIVE POLIOMYELITIS VACCINES

作　　者：郭仁[1] 陈淑范[1] 罗其胜[1] 王庆玲[1] 易红昆[1] 詹琼芬 

机构地区：[1]中国医学科学院医学生物学研究所,昆明650107

出　　处：《Zoological Research》1999年第4期241-246,共6页动物学研究（英文）

摘　　要：选用Ⅲ型脊髓灰质炎病毒,3批活疫苗样品(WHO/Ⅲ参考制品,93/363和3J两批猴体神经毒力实验不合格的疫苗)和1株标准强毒株(Leon),脊髓内注入携带有人细胞脊髓灰质炎病毒受体基因的转基因小鼠(PVRTg21)。临床观察和组织病理学检查表明,Leon病毒的毒力极强,20log10TCID50可使100%小鼠麻痹和死亡。WHO/Ⅲ疫苗参考制品毒力最弱,55log10TCID50才能使817%小鼠麻痹和517%小鼠死亡。另两个疫苗样品的毒力都高于参考制品。各项观察指标随毒力不同而有明显差别,和猴体神经毒力实验结果一致。证明PVRTg21小鼠可用作评价脊髓灰质炎活疫苗毒力试验的动物模型,也可用于病毒学和流行病学研究。Transgenic mice carrying the human poliovirus receptor (PVRTg21) were inoculated intraspinally with 3 poliovirus vaccine type 3 (WHO/Ⅲ,93/363 and 3J) and 1 wild virus (Leon).The clinical data and pathological data indicated that Leon viruses were the most strong neurovirulent.Even a dose of 100 TCID 50 could make 100% of the Transgenic mice paralysis and death.When inoculated with 5 5 log10 TCID 50 of the vaccine reference (WHO/Ⅲ),only 87 1% of the mice paralyzed and 51 7% of the mice died,and the other 2 lots of poliomyelitis vaccine (93/363 and 3J) were higher in the neurovirulence than the vaccine reference (WHO/Ⅲ).A good correlation was found between the monkey neurovirulence test (MNVT) and the PVRTg21 mouse neurovirulence test for the 3 vaccine lots.The experimental results showed that the PVRTg21 transgenic mice should possibly be an animal model for the neurovirulence evaluation of live poliomyelitis vaccines and epidemiological surveillance.

关 键 词：脊髓灰质炎 活疫苗 神经毒力试验 转基因小鼠 

分 类 号：R512.401[医药卫生—内科学] Q785[医药卫生—临床医学]