海拔高度对大车前叶和根中抗氧化系统的影响  

Effects of Altitude on Antioxidative System in Leaves and Roots of Plantago major 

作　　者：陈雄[1] 王宗灵[1] 任红旭[1] 朱元林[2] 

机构地区：[1]兰州大学干旱农业生态国家重点实验室,兰州730000 [2]中国科学院兰州冰川冻土研究所冻土工程国家重点实验室,兰州730000

出　　处：《Acta Botanica Sinica》1999年第8期846-850,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：中国科学院冻土工程国家重点实验室开放基金

摘　　要：以甘肃马衔山（ 海拔３７５０ ｍ）自然生长的大车前（ Ｐｌａｎｔａｇｏ ｍａｊｏｒ Ｌ．） 为材料，通过检测比较不同海拔高度下大车前根和叶中抗氧化保护系统的变化，探讨植物对于高寒环境的适应机理。结果表明，随着海拔的升高（ 从１６００ ｍ～３３００ ｍ） ，根中还原型谷胱甘肽（ＧＳＨ）含量增加，过氧化物酶（ＰＯＸ）和超氧化物歧化酶（ＳＯＤ） 活性显著升高，叶中的ＰＯＸ和ＳＯＤ活性则逐渐降低。高海拔区（３３００ ｍ）根中出现了特异性酶带ＰＯＸ５ 和ＳＯＤ８。这种趋势可能有利于根的越冬，而对叶片的越冬不利。Antioxidative systems were investigated in leaves and roots of Plantago major L. which were collected from Mahan Mountain at different altitudes. The content of glutathione (GSH) in reduced form was increased in leaves and roots with the increasing altitude. The activities of peroxidase (POX) and superoxide dismatase superoxide dismutase (SOD) in roots also increased as the altitude was increased, while that in leaves they declined at higher altitude. Analyses of isozymes of SOD and POX in leaves and roots showed that high altitude (3?300 m) induced the SOD isozymes 8 and POX isozyme 5 in roots, which appeared to be required for protection against lower temperature and higher light intensity as altitude was increased. The present study suggested that high altitude had affected differently the antioxidative system in leaves and roots.

关 键 词：海拔 抗氧化保护系统 适应性 大车前 

分 类 号：Q948.114[生物学—植物学]