烟草中央细胞离体受精过程中雌雄核融合生活动态的记录  

The First Record of Dynamics of Male_Female Nuclear Fusion in Viable Tobacco Central Cell During in Vitro Fertilization 

作　　者：孙蒙祥 AlessandraMOSCATELLI 杨弘远[2] MauroCRESTI 

机构地区：[1]锡耶那大学环境生物学系 [2]武汉大学发育生物学研究中心,武汉430072

出　　处：《Acta Botanica Sinica》1999年第8期906-908,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：欧盟合作项目;国家自然科学基金

摘　　要：In vitro double fertilization in tobacco ( Nicotiana tabacum L.) was carried out and one of its significant events, the dynamics of bisexual nuclear fusion in the viable central cell, was observed by video_enhanced microscopy for the first time. The observation revealed that after in vitro fertilization the male nucleus was first fused with one of the polar nuclei, then the other. The whole fusion process could be finished within two seconds. Morphologically the fusion was very similar to common protoplast fusion mediated by polyethylene glycol. It also went through a series of processes, namely touch, adherence, membrane fusion and content mixture. The male nucleolus moved closely towards the female one but no further fusion was recorded although eventually a big nucleolus was observed in the primary endosperm nucleus. The technique for in vitro fertilization and the observation of the nuclear fusion process may enable us to peep at the mechanism of male and female gamete fusion.In vitro double fertilization in tobacco ( Nicotiana tabacum L.) was carried out and one of its significant events, the dynamics of bisexual nuclear fusion in the viable central cell, was observed by video_enhanced microscopy for the first time. The observation revealed that after in vitro fertilization the male nucleus was first fused with one of the polar nuclei, then the other. The whole fusion process could be finished within two seconds. Morphologically the fusion was very similar to common protoplast fusion mediated by polyethylene glycol. It also went through a series of processes, namely touch, adherence, membrane fusion and content mixture. The male nucleolus moved closely towards the female one but no further fusion was recorded although eventually a big nucleolus was observed in the primary endosperm nucleus. The technique for in vitro fertilization and the observation of the nuclear fusion process may enable us to peep at the mechanism of male and female gamete fusion.

关 键 词：烟草 离体受精 中央细胞 核融合 

分 类 号：Q944.44[生物学—植物学] S572.032[农业科学—烟草工业]