磁场对防风体细胞胚发生发育和亚显微结构的影响  

The Effect of Magnetic Field on Somatic Embryogenesis and Embryoid Growth as well as Changes of Sub microscopic Structure of Saposhnikovia divaricata

作　　者：慈忠玲[1] 

机构地区：[1]内蒙古林学院沙漠治理系,呼和浩特010019

出　　处：《植物生理学报（0257-4829)》1999年第3期309-312,共4页Acta Phytophysiologica Sinica

摘　　要：Calli were induced from the cuttings of test tube seedlings of Saposhnikovia divaricata (Turcz.)Schischk.cultured on MS medium containing 2,4 D 0.5 mg/L. After being transferred to a new medium without 2,4 D, the calli were put in the 160 kA/m magnetic field to induce embryogenesis. Magnetic field had no influence on the frequency of embryogenesis, but made the growth of the embryoids slower than control(PlateⅠ 6,7). It restrained the growth of cotyledon embryoids and shoot apex extension in small test tube seedlings, but accelerated growth of the roots, so resulted in seedlings looking like “todpoles”(PlateⅠ 10,11), which could develop into normal seedlings with better developed roots than those of the control group(PlateⅠ 9,12). The seedlings could be deformed when put in the magnetic field for a long time(PlateⅠ 13). Sub microscopic structural changes during the treatment were observed. It was noticed that the cell vacuolized rapidly, the electronic density of the cytoplasm became progressively lower, spherosome number decreased remarkably(PlateⅡ 2,4), the shape of cell nucleus changed and the chromatins seemed to be coacervating(PlateⅡ 5). But various cell organelles were still present.Calli were induced from the cuttings of test tube seedlings of Saposhnikovia divaricata (Turcz.)Schischk.cultured on MS medium containing 2,4 D 0.5 mg/L. After being transferred to a new medium without 2,4 D, the calli were put in the 160 kA/m magnetic field to induce embryogenesis. Magnetic field had no influence on the frequency of embryogenesis, but made the growth of the embryoids slower than control(PlateⅠ 6,7). It restrained the growth of cotyledon embryoids and shoot apex extension in small test tube seedlings, but accelerated growth of the roots, so resulted in seedlings looking like “todpoles”(PlateⅠ 10,11), which could develop into normal seedlings with better developed roots than those of the control group(PlateⅠ 9,12). The seedlings could be deformed when put in the magnetic field for a long time(PlateⅠ 13). Sub microscopic structural changes during the treatment were observed. It was noticed that the cell vacuolized rapidly, the electronic density of the cytoplasm became progressively lower, spherosome number decreased remarkably(PlateⅡ 2,4), the shape of cell nucleus changed and the chromatins seemed to be coacervating(PlateⅡ 5). But various cell organelles were still present.

关 键 词：防风 体细胞胚 磁场 亚显微结构 发生 发育 

分 类 号：Q947[生物学—植物学] S567.239[农业科学—中草药栽培]