λDNA导入引起小麦染色体变异的研究  

Studies of chromosome variation of wheat infused with λDNA

作　　者：于元杰[1] 沈法富[1] 程立 刘风珍[1] 尹承佾[1] 

机构地区：[1]山东农业大学农学系,泰安271018

出　　处：《西北植物学报》1999年第3期381-386,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家科委;山东省科委"九.五"资助项目

摘　　要：小麦种子从萌动至 ２ 叶期，用 λ Ｄ Ｎ Ａ 进行浸滴处理， Ｄ１ 代出现花粉母细胞染色体变异的植株占观察株的 ７３３％ ，染色体变异的细胞占观察细胞数的 ２８３％ 。主要有染色体落后、单价体、染色体桥、多价体、染色体多极分离，染色体螺旋化不同步，异常二分体和四分体及微核等类型，随世代增进，染色体行为趋于稳定，但不同个体间存在较大差异。这种复杂的染色体变异可能是外源 Ｄ Ｎ Ａ 在受体整合过程中的细胞学反应。rome germination to two leaf stage,wheat seeds were trickled several times with liquor of DNA.In the meiosis of pollen mother cell (PMC),chromosome variation frequency is as high as 73.3% among D 1 plant.The cell accounting for 28.3% had chromosome variation.The types of chromosome variation were lagging,univalants,bridge,multivalant,segregation with poly pole,helixing with difference step.Along with generation development,the frequency of chromosome variation decreased in meiosis cell tending to stability,but the difference is existance among individuals.This coplex chromosome variation may be caused by exenous DNA intergerate into receptor genome. Key words:

关 键 词：小麦 λDNA导入 减数分裂 染色体变异 

分 类 号：S512.103.2[农业科学—作物学]