应激抑制淋巴细胞转化的时间效应  

TIME EFFECT OF RESTRAINT STRESS INDUCED SUPPRESSION OF LYMPHOCYTE TRANSFORMATION

作　　者：邱一华[1] 彭聿平[1] 程纯[2] 戴丽[1] 

机构地区：[1]南通医学院生理学教研室 [2]微生物学教研室,南通226001

出　　处：《生理学报》1999年第4期477-480,共4页Acta Physiologica Sinica

摘　　要：本研究使用束缚方法使大鼠接受应激刺激， 然后分别取大鼠应激３ 、６ 、１２、１８ ｈ 和解除束缚后１２ 、２４ 、４８ 、７２ 、９６ ｈ 的淋巴结、脾脏提取物和血清， 与刀豆素Ａ 同时加入正常大鼠肠系膜淋巴结细胞悬液中孵育７２ ｈ 后用噻唑蓝（ＭＴＴ） 比色分析法检测肠系膜淋巴结细胞的转化（ 以光密度ＯＤ 值表示） ， 来观察应激抑制淋巴细胞转化作用的出现和消失过程。结果如下：（１） 应激３ 和６ ｈ 大鼠的淋巴结、脾脏提取物和血清对淋巴细胞的转化都没有明显的影响；（２）应激１２ ｈ， 大鼠的淋巴结、脾脏提取物和血清都出现对淋巴细胞转化的明显抑制作用， 应激１８ｈ 的作用更强；（３） 淋巴结和脾脏提取物对淋巴细胞转化的抑制作用一直持续至束缚解除后９６ ｈ 仍然较强， 但血清对淋巴细胞转化的抑制作用在束缚解除后逐渐减弱， 至９６ ｈ ， 作用基本消失。上述结果说明， 大鼠束缚应激１２ ｈ 以上， 其淋巴结、脾脏和血清同步出现对淋巴细胞转化的抑制作用； 这种抑制作用即使在束缚解除后仍能存在较长时间； 血清的抑制作用时间短于淋巴结和脾脏的抑制作用时间。结果提示， 束缚应激可引起淋巴组织产生一种能抑制免疫功能的应激免疫抑制蛋白（ＩＳＰＳ） ，In the present study, restraint, which caused no direct tissue damage, was selected as a method of stress to investigate time effect of stress induced suppression of lymphocyte transformation in rats. The lymph node and spleen extract or the serum from rats restrained for 3, 6, 12, 18 h or 12, 24, 48, 72, 96 h after restraint, with concanavalin A (Con A), was added to the lymphocyte suspension of rat mesenteric lymphnode and was ncubated for 72 h. The optical density (OD) value indicating the level of lymphocyte transformation was determined by colorimetric assay of methylthiazol tetrazolium bromide (MTT). The results are as follows. (1) The lymph node and spleen extract or the serum from the rats restrained for 3 or 6 h did not markedly affect the lymphocyte transformation. (2) The lymph node and spleen extract or the serum from the rats restrained for 12 h or 18 h significantly inhibited the lymphocyte transformation and stronger inhibition occurred at 18 h. (3) Up to 96 h after restraint, the suppressive effect of the lymph node or spleen extract on the lymphocyte transformation still existed, but the suppression of the serum on the lymphocyte transformation almost disappeared. These results suggest that the restraint of rats may cause lymphoid tissues to produce some immune suppressive protein of stress, which is released into blood leading to a generalized suppression of cellular immunity.

关 键 词：应激 淋巴细胞转化 时间效应 

分 类 号：R392.12[医药卫生—免疫学] R363.1[医药卫生—基础医学]