银屑病患者骨髓CD34^+细胞HLA-C和PDCD1表达的研究  

The study of HLA-C and PDCD1 in bone marrow CD34^+ cells from patients with psoriasis

作　　者：李俊琴[1] 侯瑞霞[1] 尹国华[1] 张静[2] 张开明[1] 

机构地区：[1]太原市中心医院皮肤科,山西太原030009 [2]太原市中心医院内科,山西太原030009

出　　处：《临床皮肤科杂志》2011年第3期132-135,共4页Journal of Clinical Dermatology

基　　金：国家自然科学基金(30771940;30972657);山西省自然科学基金(2009011053-2)资助项目

摘　　要：目的:研究HLA-C(histocompatibility complex class C)和PDCD1(programmed cell death 1)在银屑病患者骨髓CD34^+细胞中的表达,以揭示银屑病患者造血干细胞的活性。方法:采用免疫磁珠法分离CD34^+细胞,RT-PCR法分别检测HLA-C、PDCD1的mRNA表达。结果:银屑病患者骨髓CD34^+细胞HLA-CmRNA表达水平高于正常对照组(P<0.05),PDCD1 mRNA表达在银屑病组与正常对照组间比较无统计学意义(P>0.05),未发现HLA-C mRNA表达水平与银屑病皮损面积和疾病活动性指数(PASI)评分有直线相关性。结论:影响CD34^+细胞发育分化的某些基因在银屑病患者骨髓CD34^+细胞中表达异常,这些基因表达水平的异常可能引起骨髓造血干细胞活性异常,并参与银屑病的发病。Objective： To reveal the biological activity of hematopoietic cells in the patients with psoriasis through investigating the expression of HLA-C and programmed cell death 1 （PDCD1） on bone marrow CD34^＋ cells. Methods： Bone marrow CD34^＋ cells were isolated from patients with psoriasis and normal persons by magnetic cell sorting system. Expression of mRNA for HLA-C and PDCD1 was analyzed using reverse transcriptase-polymerase chain reaction （RT-PCR）. Results： The expression of HLA-C in bone marrow CD34^＋ cells from patients with psoriasis was significantly higher than that from normal eontrols（P 〈 0.05）, while the expression of PDCD1 mRNA in psoriasis patients and normal controls had no significant difference （P 〉 0.05）,The level of HLA-C expression showed no positive linear correlation with PASI score. Conclusions： There is an abnormal gene expression in the bone marrow CD34^＋ cells from patients with psoriasis, which may influence the differentiation and development of hematopoietic cells and related to the pathogenesis of psoriasis, perhaos olav a oivotal role in the nathozenesis of nsoriasis.

关 键 词：银屑病 骨髓 CD34^+ 细胞 HLA-C PDCD1 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]