一种简易的免疫PCR方法的建立  

A Simplification of the Immuno PCR Assay.

作　　者：徐平西[1] 肖华胜[1] 鞠躬[1] 

机构地区：[1]第四军医大学神经科学研究所,西安710032

出　　处：《生物化学与生物物理进展》1999年第4期396-398,共3页Progress In Biochemistry and Biophysics

摘　　要：免疫 Ｐ Ｃ Ｒ 为一种高敏感度检测抗原的新技术， 操作程序大多沿袭 Ｅ Ｌ Ｉ Ｓ Ａ 方法． 用戊二醛作连接剂， 将蛋白质高效率包被在普通 Ｐ Ｃ Ｒ 管内壁， 使免疫及 Ｐ Ｃ Ｒ 反应用普通 Ｐ Ｃ Ｒ 仪得以在管中连贯地进行． 实验结果表明，标准曲线的线性关系好， 与 Ｅ Ｌ Ｉ Ｓ Ａ 方法比较敏感度高出约１０ ５ ． 这一改良法的建立， 可望促进免疫 Ｐ Ｃ ＲThe Immuno PCR antigen detection system has been developed for several years as a modefication of the ELISA method. But only a few of laboratorise applied this high sensitive quantitative assay , since it needs special apparutus or complicated operations.A new procedure of Immuno PCR was established, which coats antibody or antigien on PCR tubes with 0 8% glutaraldehyde instead of on the wells of titer plates with alkine carbonate buffer. The assay could be coducted with simple handlings and ordinary apparutus, and a high sensitivity is obtained up to 10 5 times higher than that of ELISA in S 100 antigen detection.

关 键 词：免疫PCR 包被 戊二醛 PCR管 

分 类 号：R446.61[医药卫生—诊断学]