微量量热法研究左金丸与反左金丸对肠道菌群生长代谢的影响  

Effect of Zuojin Pill and Fanzuojin Pill on the Growth Metabolism of Enterobacteria by Microcalorimetry

作　　者：程丹红[1,2] 赵艳玲[1] 杨宏博[1,3] 王伽伯[1] 贾雷[1,2] 肖小河[1] 

机构地区：[1]解放军中药研究所,解放军302医院,北京100039 [2]成都中医药大学药学院,成都611137 [3]昆明理工大学生命科学与技术学院,昆明650224

出　　处：《中国中西医结合杂志》2011年第2期209-212,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划资助项目(973计划)(No.2007CB512607);国家自然科学基金资助项目(No.30772740)

摘　　要：目的从生物热动力学角度研究左金丸、反左金丸对肠道菌群生长代谢的影响,验证其客观差异性,探讨类方研究的新方法。方法采用微量量热法测定小鼠肠道菌群在不同浓度的左金丸、反左金丸作用下的生长代谢热谱曲线,以特征参数生长速率常数(k)、最大发热功率(Pmax)及达峰时间(Tp)、总产热量(Qt)和细菌抑制率(I)为指标,综合评价左金丸与反左金丸的活性差异。结果左金丸与反左金丸作用下的肠道菌群,随着药液浓度的增加,达峰时间Tp均延长;而左金丸与反左金丸对肠道细菌生长速率常数k值的影响却不同,与空白对照组比较,二者作用下的k值均减小,即两者均抑制肠道细菌的生长;由抑制率I可知,左金丸抑菌活性大于反左金丸。结论左金丸与反左金丸的抑菌活性存在客观差异性;微量量热法具有实时、在线、灵敏的特点,可快速、准确地评价类方的活性差异。Objective To study the effects of Zuojin Pill and Fanzuojin Pill （ZJP and FZJP） on the growth metabolism of enterobacteria from the biothermodynamics viewpoint,and to objectively validate the difference between the two drugs for investigating a new way in congener Chinese drugs research. Methods The growth metabolism power-time curves of enterobacteria affected by ZJP and FZJP in different concentrations were obtained by microcalorimetry. And the activities of the two drugs were assessed depending some related characteristic parameters,including growth rate constant （k）,maximum output power （Pmax）,peak time （Tp） ,maximum heat-output （Qt）,and bacteria inhibition rate （I）. Results With the drugs＇ concentration increasing,Tp was prolonged,k value reduced in enterobacteria,i.e.,the two drugs both inhibited the growth of enterobacteria in different extents. Comparison of inhibiting rates showed that the bacteriostasis activity of ZJP was greater than that of FZJP. Conclusions There was objective difference in activities of inhibiting enterobacteria between ZJP and FZJP. Microcalorimetry could express the features at real-time,on-line and sensitively. It was able to determine the activity of drugs accurately and quickly in appraising congener Chinese drugs,like ZJP and FZJP,by microcalorimetry.

关 键 词：微量量热法 左金丸 反左金丸 肠道菌群 

分 类 号：R285[医药卫生—中药学]