云南省玉米大斑病菌的RAPD分析  

Phylogenetic analysis on Exserohilum turcicum isolates from Yunnan province by RAPD

作　　者：王利智[1,2] 吴景芝[2] 康志钰[1] 何月秋[1,2] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学教育部农业生物多样性与病虫害控制重点实验室,云南昆明650201

出　　处：《云南大学学报（自然科学版）》2011年第2期244-248,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省支撑计划项目资助(2006NG19);云南省行业体系项目资助

摘　　要：应用7条随机引物对采自云南省不同玉米产区的玉米大斑菌81个菌株的DNA进行了RAPD分析.结果表明,这些菌株共产生60条谱带,其中43条为多态性带,占71.7%.利用UPGMA法对DNA扩增图谱进行聚类分析,以遗传距离0.50为阈值,81个菌株可分为5个遗传群体,表明云南省玉米大斑病菌中具有丰富的种内遗传多样性.然而,这种分子标记所划分的群体与菌株来源地及致病类型间没有直接关系.In order to study the genetic variation among Exserohilum turcicum isolates,the genomic fingerprints of 81 isolates of E.turcicum collected from different corn-growing areas of Yunnan province are analyzed by using 7 RAPD primers.The results show that there are 60 bands amplified and among them,43 bands are polymorphic（71.7%）.By using UPGMA,the 81 isolates are clustered into 5 groups based on the threshold of the genetic distance which is 0.50,indicat that E.turcicum isolates are diverse in Yunnan province.However,there is no direct relationship between the genetic groups divided by RAPD and their geographic origins and the pathogenic types.

关 键 词：玉米大斑病菌 RAPD 遗传多态性 云南省 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]