四川省MSM人群HIV-1原发耐药性分析  

Analysis on Primary Drug Resistance of HIV-1 Infected Men Having Sex With Men in Sichuan Province

作　　者：袁丹[1] 秦光明[1] 肖林[2] 吕超[3] 牛安民 魏东兵[1] 冯燎[1] 刘红露[1] 

机构地区：[1]四川省疾病预防控制中心艾滋病确证中心实验室,四川成都610041 [2]凉山州疾病预防控制中心卫生检验所 [3]中国疾病预防控制中心艾滋病参比室 [4]北京科卫临床诊断试剂有限公司

出　　处：《预防医学情报杂志》2011年第3期169-172,共4页Journal of Preventive Medicine Information

基　　金：中国第四轮全球基金艾滋病项目;项目编号:CHN-405-G05-H

摘　　要：目的分析四川省男男同性恋(MSM)人群中未经抗病毒治疗的人类免疫缺陷病毒I型(HIV-1)感染者的耐药突变的流行情况。方法采集成都、绵阳和达州3个地区MSM中未经治疗的HIV-1感染者血样,进行核酸提取、PCR及基因测序,对所得结果登录美国斯坦福大学HIV耐药数据库进行在线分析确定耐药突变情况。结果 77例样本中,11例存在蛋白酶抑制剂次要相关突变,4例存在核苷类逆转录酶抑制剂相关突变,5例存在非核苷类逆转录酶抑制剂相关突变,其中1例潜在耐药。结论四川省MSM中未经治疗的HIV-1感染者少数存在原发耐药相关基因变异,未发现新耐药毒株,原发耐药株仍处于较低流行状态,感染者在抗病毒治疗开始前应定期检测耐药基因突变情况以指导用药。Objective To understand the HIV-1 drug resistant mutation rate in HIV-1 infected men having sex with men（MSM） in Sichuan province.Methods The blood samples were taken from the HIV-1 infected MSM who received no treatment previously in Chengdu,Mianyang and Dazhou to extract nucleic acid,conduct PCR and gene sequencing. The results were analyzed with HIVdb-Drug Resistance Algorithm and genotypic resistance mutations were determined to particular anti-HIV drugs.ResultsAmong 77 samples,the sequences of 11 samples displayed minor resistant mutations in the protease gene.Genotypic resistance to at least one nucleoside reverse transcriptase inhibitors（NRTI） was observed in 4 sequences,and resistance to at least one non-nucleoside reverse transcriptase inhibitors（NNRTI） was observed in 5 sequences.ConclusionThe primary HIV-1 drug resistance mutations was observed in a few HIV infected MSM in Sichuan,no drug resistant strain was detected and the prevalence of the original drug resistant strain was low.Careful and systematic detection of the mutation of drug-resistant gene before anti-viral therapy is necessary to guide anti-HIV therapy.

关 键 词：人类免疫缺陷病毒I型 原发耐药 耐药突变 男男同性恋 

分 类 号：R512.91[医药卫生—内科学]