消痰散结方对鼠胃移植瘤相关炎性因子表达的影响  

Effect of Xiaotansanjie Decoction on Expression of Inflammatory Factors Correlated to Gastric Tumor Xenografts In Mice

作　　者：巨大维[1] 魏品康[1] 孙大志[1] 修丽娟[1] 陆烨[1] 刘煊[1] 

机构地区：[1]第二军医大学附属长征医院,上海200003

出　　处：《中国中医急症》2011年第3期407-409,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然基金重大研究计划面上项目(No.90709044);上海长征医院"三重三优"学科建设重大项目(2006-2010年)

摘　　要：目的观察消痰散结方对小鼠胃移植瘤和瘤旁胃组织中炎性因子白细胞介素6(IL-6)和白细胞介素8(IL-8)表达的调节作用,探讨消痰散结方抑瘤、防复发的部分作用机制。方法 40只昆明小鼠随机分为正常组、模型组、喃氟啶组、消痰散结方组;除正常组外,其余各组通过移植S180瘤块,建立小鼠胃移植瘤模型;实验3周后,剥取肿瘤,称取瘤质量,计算抑瘤率;运用RT-PCR及ELISA方法分别检测瘤及瘤旁胃组织中IL-6mRNA及IL-8蛋白表达。结果正常胃组织-瘤旁胃组织-瘤组织中IL-6、IL-8表达呈递增趋势;消痰散结方较喃氟啶可明显抑制肿瘤生长、下调肿瘤及瘤旁胃组织中IL-6、IL-8的表达。结论消痰散结方可明显下调胃肿瘤及瘤旁胃组织中IL-6、IL-8表达,是其抑瘤、防复发的可能机制之一。Objective：To explore the mechanisms of Xiaotansanjie Decoction in inhibiting the tumor growth and preventing recurrence by testing expressions of IL-6 and IL-8 in gastric tumor xenografts and gastric tissue adjacent to tumor in mice.Methods：40 Kunming mice were randomly divided into the normal group,the model group,the FT-207 group and the Xiaotansanjie Decoction group.The gastric tumor xenografts model was established except normal group by transplanting S180 tumor block.The experiment was continuous for 3 weeks.The weight of tumor xenografts was measured and the inhibition rate was calculated.The expressions of IL-6mRNA and IL-8 were respectively tested by RT-PCR and ELISA methods.Results：The expressions of IL-6 and IL-8 were increased progressively in the normal gastric tissue and the gastric tissues beside tumor and tumor.The restrain degree of Xiaotansanjie Decoction,which was significantly in the expression of IL-6 and IL-8 was more than that of FT-207.Conclusion：Xiaotansanjie Decoction can decrease the expressions of IL-6 and IL-8 in tumor xenografts and gastric tissue adjacent to tumor.It is one of the mechanisms of Xiaotansanjie Decoction in inhibiting the tumor growth and preventing recurrence.

关 键 词：消痰散结方 胃肿瘤 肿瘤移植 白细胞介素6 白细胞介素8 

分 类 号：R285.5[医药卫生—中药学]