两个ADP-葡萄糖焦磷酸化酶α亚基基因在甘薯中的超量表达  

Over Expression of Two α-subunit Genes of ADP-glucose Pyrophosphorylase in Sweet Potato

作　　者：郑雪莲[1] 张聪[2] 吴洁[2] 蒲志刚[2] 王大一[3] 阎文昭[2] 

机构地区：[1]电子科技大学生命科学与技术学院,四川成都610054 [2]四川省农业科学院生物技术核技术研究所,四川成都610066 [3]四川省农业科学院作物研究所,四川成都610066

出　　处：《西南农业学报》2011年第1期29-33,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)(2006AA100107);"十一五"国家科技支撑计划(2007BAD78B00)

摘　　要：ADP-葡萄糖焦磷酸化酶(AGPase)催化ADP-葡萄糖合成反应,是淀粉生物合成的限速酶之一。为研究甘薯AGPase与块根淀粉合成的关系,从高淀粉甘薯品种川薯34中克隆了AGPase基因的两个α亚基编码序列AGPa1和AGPa2;构建植物表达载体pC-AGPa1和pC-AGPa2后经根癌农杆菌EHA105介导分别导入甘薯品种西成薯007;获得表达了GUS的7株AGPa1和5株AGPa2转基因甘薯植株。RT-PCR和AGPase活性检测表明,AGPa1和AGPa2基因在转基因植株中表达水平高于对照,并引起甘薯叶片中AGPase活性显著增加。ADP-Glucose pyrophosphorylase（AGPase） is the key enzyme responsible for the production of adenosine 5＇-diphosphase glucose（ADPGlc）,which is the first committed step in the biosynthesis of starch.To investigate the effect of AGPase on starch synthesis in sweet potato,we isolated two α-subunit genes of AGPase,AGPa1 and AGPa2,from high-starch sweet potato（Ipomoea batatas（L）Lam.） Chuanshu 34.Afterwards plant expression vectors pC-AGPa1 and pC-AGPa2 containing AGPa1 and AGPa2 were introduced into sweet potato Xicheng 007 by Agrobacteria-mediated transformation,respectively.After PCR analysis and GUS-staining,seven AGPa1 and five AGPa2 transgenic plants were obtained.Furthermore,RT-PCR analysis indicated that AGPa1 and AGPa2 mRNA accumulation in transgenic plants was higher than that of wild type plants,respectively.And the AGPase activities of AGPa1 and AGPa2 transgenic plants were also higher than those of wild type plants consistently.This was an important step toward producing new starch crops for fuel ethanol industry.

关 键 词：甘薯(Ipomoea BATATAS (L.) Lam.) ADP-葡萄糖焦磷酸化酶 淀粉合成 遗传转化 

分 类 号：S531[农业科学—作物学]