七氟醚预处理对心肌缺血再灌注大鼠心肌线粒体蛋白质组学双向凝胶电泳分析  

Proteomics Analysis of Sevoflurane Delayed Preconditioning on Rat Myocardium Mitochondria with Two Dimensional Polyacrylamide Gel Electrophoresis

作　　者：丁丽萍[1] 冉珂[1] 肖艳英[1] 常业恬[1] 

机构地区：[1]中南大学湘雅二医院麻醉科,湖南长沙410011

出　　处：《医学临床研究》2011年第2期220-223,共4页Journal of Clinical Research

基　　金：湖南省自然科学基金项目（10JJ4021）

摘　　要：[目的]研究七氟醚预处理对缺血再灌注心肌保护的线粒体相关蛋白的变化.[方法]采用双向凝胶电泳技术对七氟醚预处理（S组）与对照组（C组）大鼠心肌线粒体蛋白质进行分离和比较分析.[结果]S组与C组相比,有21个蛋白质表达发生了改变,其中表达增强的有17个蛋白,表达降低的有4个蛋白.[结论]这些差异表达的蛋白质可能参与了七氟醚预处理对心肌的保护作用.[Objective] To explore the changes of mitochondria related proteins for myocardal protection of sevoflurane delayed preconditioning in ischemic reperfusion rats. [Methods] Two dimensional polyacrylamide gel electrophoresis（2-DE） was used to separate and compare the rat myocardium mitochondria in sevoflurane delayed preconditioning group（group S） and control group（group C）. [Results] Compared with group C, the expression of 21 proteins changed in group S, in which the expression of 17 proteins increased and the expression of 4 proteins decreased. [Conclusion] These differential expression proteins may be involved in the myo cardiai protection of sevoflurane delayed preconditioning.

关 键 词：线粒体 心脏 蛋白质类 醚类/药理学 电泳 琼脂凝胶 大鼠 

分 类 号：R971.2[医药卫生—药品]