PCR-RFLP法快速测定种猪氟烷基因型及其使用优点  

Determination of Halothane Genotype Pigs by PCR-RFLP Rapidly and its Using Advantages

作　　者：祁永旺[1] 王昕陟[1] 侯华[1] 张翠[1] 王金旭 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]沈阳市畜牧兽医研究所,沈阳110034

出　　处：《饲料博览》2011年第2期27-30,共4页Feed Review

基　　金：国家科技部十一五科技支撑项目(2007BAD71B011-03)

摘　　要：从猪毛中提取DNA,用特异性引物进行PCR-RFLP扩增法对沈阳市畜牧兽医研究所种猪场种猪进行氟烷基因检测。结果表明,在测定的长白、大约克、杜洛克种猪中,发现长白猪HaLn基因的基因频率较高,为7.5%、大约克为2.1%、杜洛克为2.0%。所有种猪不含易引起猪应激综合征的氟烷基因隐形纯合子(nn)。用猪毛进行PCR扩增不仅成本低、速度快,且准确性高、取样方便,可大批量地处理样品。从仔猪开始至成年种猪,均可用毛样进行基因诊断,从而有效防止氟烷基因隐形纯合子(nn)的出现,加快种猪的改良步伐。Extracted from the pig using specific primers for PCR-RFLP amplification of Shenyang Institute of Animal breeding farms. The result showed that the gene landrace Haln gene frequencies was higher 7.5%, Yorkshire 2.1%, Duroc 2.0% in the determination of the landrace, Yorkshire and Duroc. There was no homozygous halothane gene（nn） which lead porcine stress syndrome. There was not only low cost, fast and high accuracy, convenience sampling, sample handling large quantities at one time by PCR amplified with the pig hair. To diagnosis gene by pig fair from piglets to adult breeding. Thus prevent effectively homozygous halothane gene（nn） appearance, accelerating the pace of improvement of breeding pig.

关 键 词：氟烷基因 PCR-RFLP扩增法 猪应激综合征 

分 类 号：S828[农业科学—畜牧学] S856.1[农业科学—畜牧兽医]