木蹄层孔菌多糖对免疫抑制小鼠免疫功能及细胞因子产生的影响  

Effects of Fomes fomentarius polysaccharides on immune function and cytokines production of immunosuppressive mice

作　　者：周桂保[1] 高慧灵[2] 丁佩娥[1] 张群[1] 余传林[1] 雷林生[1] 

机构地区：[1]南方医科大学药学院,广东广州510515 [2]包头市第四医院药剂科,内蒙古包头014030

出　　处：《广东药学院学报》2011年第1期60-64,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金(7005189)

摘　　要：目的探讨木蹄层孔菌多糖(Fomes fomentarius polysaccharides,FFP,木蹄多糖)对免疫抑制小鼠单核/巨噬细胞吞噬功能、体液免疫功能及T淋巴细胞分泌白介素2(IL-2)、干扰素γ(IFN-γ)的影响。方法环磷酰胺(15 mg/kg)皮下注射,连续7 d,建立免疫抑制小鼠模型,碳粒廓清法检测单核/巨噬细胞的吞噬功能,兔红细胞免疫法检测小鼠体液免疫功能,显微镜计数法检测外周血白细胞总数,刀豆素A(Con A)刺激法检测T淋巴细胞分泌细胞因子的能力,ELISA法检测IL-2、IFN-γ的含量。结果 FFP(200、100 mg/kg)能显著提高免疫抑制小鼠单核/巨噬细胞的吞噬功能,促进免疫抑制小鼠抗兔红细胞特异性抗体(溶血素)的生成,增加免疫抑制小鼠外周白细胞总数,增强免疫抑制小鼠脾细胞在Con A刺激下产生IL-2和IFN-γ的能力。结论木蹄多糖可提高免疫抑制小鼠非特异性免疫和体液免疫功能,增强免疫抑制小鼠T淋巴细胞产生IL-2及IFN-γ的能力。Objective To investigate the effects of Fomes fomentarius polysaccharides （FFP） on humoral immune response,phagocytic activities of mononuclear macrophages and production of interleukin-2 （IL- 2） and interferon-γ（IFN-5,） by T lymphocytes in immunosuppressive mice. Methods Immunosuppressive mice model were established by subcutaneous administration of cyclophosphamide （15 mg/kg ） for 7 consecutive days. Phagocytic activity of mononuclear macrophages was determined with carbon clearance test. Humoral immunity was evaluated with the production of specific antibody （haemolysin） against rabbit red blood cells （RRBC）. Peripheral white blood ceils was counted under microscope. Cytokines production of T lymphocytes was induced by concanavalin A （Con A）. The amounts of IL-2 and IFN-＇y were measured with ELISA assay. Results It was found that FFP （200 and 100 mg/kg）significantly enhanced the phagocytic activity of mononuclear macrophages, increased the level of serum hemolysin induced by RRBC immunization, raised the total number of peripheral white blood cells and promoted the production of IL-2 and IFN-γ by T lymphocytes in immunosuppressive mice. Conclusion FFP can enhance non-specific immune function and humoral immune response of immunosuppressive mice, and promote production of IL-2 and IFN-γ by T lymphocytes in the mice.

关 键 词：木蹄层孔菌 多糖 免疫抑制 碳粒廓清 溶血素 细胞因子 

分 类 号：R285.5[医药卫生—中药学]