小檗碱对高脂-高盐-果糖诱致大鼠高血压性肾损伤的保护作用  

The protecting effects of berberine on the hypertensive renal impairment in rats fed by enriched high fat-salt-fructose diet

作　　者：蔡颖[1] 齐春丽[1] 吴铎[1] 郜蕊[1] 吕俊华[1] 

机构地区：[1]暨南大学药学院药理学教研室,广东广州510632

出　　处：《广东药学院学报》2011年第1期65-69,共5页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的探讨小檗碱对高血压性肾损伤模型大鼠肾脏的保护作用。方法 SD大鼠50只,随机分为正常组和模型组。除正常组大鼠外,其余大鼠均给予高脂-高盐饲料喂养,并每天交替饮用糖盐水(质量分数为糖4%、盐1%)和果糖水(质量分数为6%)8周。根据血压情况,随机将高血压的动物分为模型对照组(蒸馏水)、卡托普利(25 mg/kg)组、小檗碱高剂量组(300 mg·kg-1)和低剂量组(100 mg·kg-1)。继续给予高脂-高盐-果糖饲养,同时,分别灌胃给予上述药物处理4周。分别在0、4、8、10、12周末测定血压,药物处理4周后,留取尿液测定尿蛋白含量,取血测定血脂、血肌酐和血尿素氮,并在光镜下观察肾脏病理学变化。结果小檗碱处理4周,明显降低模型大鼠的血压和血胆固醇(TG)、三酰甘油(TC)、低密度脂蛋白(LDL)水平(P<0.01或P<0.05),升高高密度脂蛋白(HDL-C)水平(P<0.01),同时降低尿蛋白(U-Pro)、血肌酐(Cr)和血尿素氮(BUN)水平,降低肾脏组织Na+-K+-ATP酶含量(P<0.01),改善肾小球肥大,降低肾小球直径,减少肾脏出血。结论小檗碱对高血压性肾损伤模型大鼠具有降压、调脂、改善肾功能的作用及保持肾结构完整性的作用。Objective Investigate the protecting effects of berberine on this model. Methods 50 SD rats were randomly divided into normal group and model group. All rats of the model group were fed by enriched high fat-salt-fructose diet for 8 weeks. On the basis of animal blood pressure, hypertensive rats were randomly divided into mode/ control group （ distilled water）, captopril group （ 25 mg ·kg^-1 ）, berberine high dose group（300 mg ·kg^-1） and low dose group（ 100 mg· kg^-1）. These rats were fed by further enriched high fat-salt-fructose diet and treated by intragastric administration of related drugs for 4 weeks. Blood pressure was determined at 0,4,8,12 weekend, and after 4 weeks of drugs treatment, getting urine to determine urine protein, taking blood serum to determine blood fat, blood urea nitrogen and serum creatinine. The contents of Na^-K＋-ATPase in kidney tissues were determined also. Morphological changes of kidney were observed by light microscope. Results Treated with berberine for 4 weeks, elevated blood pressure and heightened levels of blood fat （TG,TC,LDL） in model rats were depressed significantly（P 〈0.01 or P 〉 0.05 ）, and lowered HDL-C was elevated significantly （ P 〈 0.01 ） ; the levels of urine protein, blood urea nitrogen and serum creatinine as well as content of Na ＋ -K ＋ -ATPase in kidney tissues were lowered significantly （ P 〈 0. 01 ） ; glomerular diameter and hypertrophia could be improved and degree of hemorrhage became extenuation. Conclusions Berberine can lower hypertension and regulate hyperlipemia,improve renal function and morphological integrity for hypertensive renal impairment model rats fed by enriched high fat-saltfructose diet.

关 键 词：小檗碱 高血压 血脂 肾脏功能 ATP酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]