广东人群药物代谢酶CYP2C9基因A895G位点多态性研究  

The study on the polymorphism of A895G of drug metabolizing enzyme CYP2C9 gene in Guangdong population

作　　者：何震宇[1] 黄国庆[2] 潘伟[1] 崔炳权[1] 杨红[1] 

机构地区：[1]广东药学院生物化学与分子生物学系,广东广州510006 [2]广东药学院

出　　处：《广东药学院学报》2011年第1期91-93,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金(034051);广东省医学科研基金(A2008308);广东药学院科研启动基金(2007jcx02)

摘　　要：目的调查广东人群CYP2C9基因第6外显子895A>G位点多态性情况。方法 PCR扩增跨越该位点的DNA片断,用VpaK11BI酶切PCR产物,通过PAGE-银染判断基因型。结果在受检的252例广东人中,共检出3例A895/G895杂合子,余者皆为野生型纯合子A895/A895。结论广东人群存在CYP2C9895G即CYP2C9*16等位基因,其频率为0.006。本研究为广东人群使用CYP2C9底物药提供了参考依据。Objective To investigate the polymorphism of A895G in exon 6 of CYP2C9 gene in Guangdong population. Methods The DNA fragments spanning the loci A895G in exon 6 of CYP2C9 gene were amplified by polymerase chain reaction,the PCR products were subjected to VpaK11BI digestion, then the genotypes were determined with polyaerylamide gel electrophoresis （PAGE） and sliver-staining. Results Three individuals were heterozygotes （A895/G895） in 252 individuals studied, others were wild-type homozygotes. Conclusion There was CYP2C9 895G namely CYP2C9 ＊ 16 allele in Guangdong population, whose frequency was 0. 006. This study has provided some reference for the appropriate usage of drugs metabolized by CYP2C9.

关 键 词：药物代谢酶 CYP2C9 多态性 广东人群 

分 类 号：R346[医药卫生—基础医学]