流动注射-抑制化学发光法测定克百威  

FI-Inhibitory Chemiluminescence Determination of Carbofuran

作　　者：贾风燕[1] 刘振波[1] 王荣[1,2] 刘永明[1] 

机构地区：[1]烟台大学化学生物理工学院,烟台264005 [2]烟台万华超纤股份有限公司,烟台264002

出　　处：《理化检验（化学分册）》2011年第2期129-132,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然基金(20973149)

摘　　要：基于在碱性介质中,克百威抑制鲁米诺-过氧化氢-叶绿素铜钠体系的化学发光,提出了流动注射-抑制化学发光法测定克百威含量的方法。试验结果表明:叶绿素铜钠对克百威荧光猝灭过程是静态猝灭过程,叶绿素铜钠与克百威结合形成物质的量比为1比1的稳定配合物,平衡常数(K0)为3.41×105L.mol-1(25℃),结合距离(r)为0.39 nm。克百威质量浓度在0.08～2.00 mg.L-1范围内与其发光强度呈线性关系,方法检出限(3σ/k)为0.03 mg.L-1。此法用于克百威杀虫剂样品的分析,测得方法的平均加标回收率为101.5%。Based on the inhibitory effect of carbofuran on the CL-reaction system of luminol-H2O2-sodium copper chlorophyll in an alkaline medium,a method for the determination of carbofuran by FI-inhibitory chemiluminescence（CL） was proposed.It was found that a stable complex was formed by the reaction of sodium copper chlorophyll with carbofuran in the mole ratio of 1 to 1,the process of quenching of fluorescence of carbofuran was proved to be a process of static quenching.Equilibrium constant（K0） and combination distance（r） the complex were determined and values obtained were 3.41×105L·mol-1 and 0.39 nm respectively.Linear relationship was found between values of CL intensity and mass concentration of carbofuran in the range of 0.08 to 2.0 mg·L-1,and detection limit（3σ/k） of the method found was 0.03 mg·L-1.The proposed method was used in analysis of carbofuran in secticide samples,giving values of average recovery of 101.5%.

关 键 词：流动注射 抑制化学发光法 鲁米诺 叶绿素铜钠 克百威 

分 类 号：O657.39[理学—分析化学]