Biodegradation of acrylamide by Enterobacter aerogenes isolated from wastewater in Thailand  

作　　者：Kanokhathai Buranasilp Jittima Charoenpanich 

机构地区：[1]Biological Science Program and Center of Excellence for Innovation in Chemistry (PERCH-CIC),Faculty of Science,Burapha University [2]Department of Biochemistry,Faculty of Science,Burapha University [3]Environmental Science Program and Center of Excellence on Environmental Health,Toxicology and Management of Chemicals (ETM-PERDO),Faculty of Science,Burapha University

出　　处：《Journal of Environmental Sciences》2011年第3期396-403,共8页环境科学学报（英文版）

基　　金：Financial support was mainly provided to JittimaCharoenpanich from Center of Excellence on Environmental Health,Toxicology and Management of Chemicals(ETM-PERDO);partly from Faculty of Science,Burapha University;a scholarship support to Kanokhathai Buranasilp from Center of Excellence for Innovation in Chemistry (PERCH-CIC),Commission on Higher Education,Ministry of Education

摘　　要：A widespread use of acrylamide, probably a neurotoxicant and carcinogen, in various industrial processes has led to environmental contamination. Fortunately, some microorganisms are able to derive energy from acrylamide. In the present work, we reported the isolation and characterization of a novel acrylamide-degrading bacterium from domestic wastewater in Chonburi, Thailand. The strain grew well in the presence of acrylamide as 0.5% （W/V）, at pH 6.0 to 9,0 and 25℃. Identification based on biochemical characteristics and 16S rRNA gene sequence identified the strain as Enterobacter aerogenes. Degradation of acrylamide to acrylic acid started in the late logarithmic growth phase as a biomass-dependent pattern. Specificity of cell-free supernatant towards amides completely degraded butyramide and urea and 86% of lactamide. Moderate degradation took place in other amides with that by formanaide 〉 benzamide 〉 acetamide 〉 cyanoacetamide 〉 propionamide. No degradation was detected in the reactions of N,N-methylene bisacrylamide, sodium azide, thioacetamide, and iodoacetamide. These results highlighted the potential of this bacterium in the cleanup of acrylamide/amide in the environment.A widespread use of acrylamide, probably a neurotoxicant and carcinogen, in various industrial processes has led to environmental contamination. Fortunately, some microorganisms are able to derive energy from acrylamide. In the present work, we reported the isolation and characterization of a novel acrylamide-degrading bacterium from domestic wastewater in Chonburi, Thailand. The strain grew well in the presence of acrylamide as 0.5% （W/V）, at pH 6.0 to 9,0 and 25℃. Identification based on biochemical characteristics and 16S rRNA gene sequence identified the strain as Enterobacter aerogenes. Degradation of acrylamide to acrylic acid started in the late logarithmic growth phase as a biomass-dependent pattern. Specificity of cell-free supernatant towards amides completely degraded butyramide and urea and 86% of lactamide. Moderate degradation took place in other amides with that by formanaide 〉 benzamide 〉 acetamide 〉 cyanoacetamide 〉 propionamide. No degradation was detected in the reactions of N,N-methylene bisacrylamide, sodium azide, thioacetamide, and iodoacetamide. These results highlighted the potential of this bacterium in the cleanup of acrylamide/amide in the environment.

关 键 词：ACRYLAMIDE BIODEGRADATION domestic wastewater Enterobacter aerogenes 

分 类 号：O631.3[理学—高分子化学]