家猪减数分裂粗线期二价体与有丝分裂中期染色体的比较研究  

Comparative Studies on Meiosis Pachytene Bivalents and Mitosis Metaphase Chromosomes of Pig (Sus scrofa domestica)

作　　者：刘榜[1] 李奎[1] 彭中镇[1] 赵书红[1] 刘学芹[1] 周艳琴[1] 何锋[1] 

机构地区：[1]华中农业大学牧医学院动物分子生物学与育种实验室,湖北武汉430070

出　　处：《遗传》1999年第5期24-26,共3页Hereditas（Beijing)

基　　金：国家自然科学基金!39570519;瑞典国际科学基金;霍英东青年教师基金

摘　　要：以性成熟公猪睾丸和外周血为材料，采用长低渗、高氯仿卡诺固定液固定和外周血细胞培养制备减数分裂粗线期二价体和有丝分裂中期染色体，通过对二价体和有丝分裂中期染色体分裂指数和长度的比较研究，发现二价体的分裂指数和长度分别是有丝分裂中期染色体的5倍和3．42倍（1．87～5．98）；同时以12号染色体为例，比较了二价体上的染色粒结构带与有丝分裂中期染色体G－带，表明染色粒结构带比中期染色体G－带纹丰富，而与早中期G－带带织吻合。Meiotic pachytone bivalents were obtained from porcine testes using prolonged hypotonic treat ment combined with high chloroform Carnory,s fixative solution. Mitotic metaphase chromosomes were prepared ffom blood cell culture. Comparative studies on division index and length of pachytene bivalents and mitosis metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87~5.98) times longer than the latter, respectively. Chromomere maps of bivalents are more abundant than mitotic metaphase G-bands, while they are correspondent with mitotic early-metaphase G-bands. The result was found by using the chromosome 12 as a sample.

关 键 词：家猪 二价体 有丝分裂染色体 染色粒结构带 G-带 

分 类 号：Q28[生物学—细胞生物学]