μ-and m-calpain expression and activity changes following diethylstilbestrol injection in the rat anterior pituitary  

作　　者：Weijiang Zhao Zhongfang Shi Fang Yuan Guilin Li Yazhuo Zhang Zhongcheng Wang 

机构地区：[1]Neuroscience Center, Shantou University Medical College, Shantou 51504 I, Guangdong Province, China [2]Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China

出　　处：《Neural Regeneration Research》2011年第1期60-65,共6页中国神经再生研究（英文版）

摘　　要：Little is known about changes in calpain activity in the pituitary gland. In the present study,μ- and m-calpain activity changes were detected in the rat anterior pituitary following intraperitoneal injection of diethylstilbestrol. Double-immunofluorescence labeling confirmed colocalization of μ- and m-calpain in prolactin-secreting cells （lactotrophs）. Western blot analysis revealed significantly increased expression of both calpains, which accompanied upregulated cytosol and membrane zymographic activities at 12 weeks following diethylstilbestrol injection, compared with rats injected with sunflower oil. Moreover, following estrogen injection, pituitary gland pathological damage gradually worsened with increasing time. Results demonstrated that estrogen regulated calpain expression and activity, and both calpains participated in the pathophysiological processes of the pituitary gland. Ubiquitous calpain expression could serve as an effective target for anti-estrogen drugs.Little is known about changes in calpain activity in the pituitary gland. In the present study,μ- and m-calpain activity changes were detected in the rat anterior pituitary following intraperitoneal injection of diethylstilbestrol. Double-immunofluorescence labeling confirmed colocalization of μ- and m-calpain in prolactin-secreting cells （lactotrophs）. Western blot analysis revealed significantly increased expression of both calpains, which accompanied upregulated cytosol and membrane zymographic activities at 12 weeks following diethylstilbestrol injection, compared with rats injected with sunflower oil. Moreover, following estrogen injection, pituitary gland pathological damage gradually worsened with increasing time. Results demonstrated that estrogen regulated calpain expression and activity, and both calpains participated in the pathophysiological processes of the pituitary gland. Ubiquitous calpain expression could serve as an effective target for anti-estrogen drugs.

关 键 词：DIETHYLSTILBESTROL ubiquitous calpain anterior pituitary PROLACTINOMA Wistar rats 

分 类 号：S859.84[农业科学—临床兽医学] TS251.1[农业科学—兽医学]