新疆伊犁地区牧羊犬脑内亨德拉病毒感染情况  

Intracerebral Infection with Hendra Virus in Shepherd Dogs in Ili Prefecture,Xinjiang Uygur Autonomous Region,China

作　　者：王嘉佳[1,2,3] 冯裕星[1,2,3] 谢亮[2,3] 张亮[1,2,3] 何丰[1,2,3] 孙后超[1,2,3] 牟君[1,2,3] 展群岭[1,2,3] 胡永波[1,2,3] 朱丹[1,2,3] 李雷雷[2,3] 谢鹏[1,2,3] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016 [2]重庆医科大学神经科学研究中心,重庆400016 [3]重庆市神经生物学重点实验室,重庆400016

出　　处：《中国生物制品学杂志》2011年第2期133-136,145,共5页Chinese Journal of Biologicals

基　　金：国家重大科学研究计划(973计划)项目(2009CB918302);"十一五"国家高技术研究发展计划(863计划)项目(2006AA02Z196);重庆市科委科技计划项目(CSTC2008BB5238)

摘　　要：目的分析新疆伊犁地区草原放养牧羊犬脑内亨德拉病毒(Hendravirus,HeV)的感染情况。方法采用一步法实时荧光定量RT-PCR检测HeV核蛋白(N)基因片段,并验证该方法的敏感性和特异性。采用该方法对采自新疆伊犁地区草原放养、未接种HeV疫苗的101例牧羊犬脑组织进行HeV N基因片段检测。结果建立的一步法实时荧光定量RT-PCR检测HeV N基因的最低检出浓度为2.6×102 copies/μl;与其他单股负链RNA病毒,如同属且高度同源的尼帕病毒(Nipah virus,NiV)和博尔纳病病毒(Borna disease virus,BDV)等无交叉反应;101份牧羊犬脑组织样本HeV N基因的检测结果均为阴性。结论尚未发现我国新疆伊犁地区天然牧场放养牧羊犬中存在HeV感染的证据,该地区短时间内爆发HeV感染的危险性不大。Objective To investigate the intracerebral infection with Hendra virus（HeV） in shepherd dogs in Ili Prefecture,Xinjiang Uygur Autonomous Region,China.Methods A one-step real-time fluorescent quantitative RT-PCR method for nucleoprotein（N） gene fragment of HeV was developed,verified for sensitivity and specificity,and used for determination of HeV N gene in 101 brain tissue samples of uninoculated shepherd dogs bred in grassland in Ili.Results The minimum detection limit of HeV N gene by the developed method was 2.6 × 102 copies / μl.No cross reactions with other single negative strand RNA virus such as Nipah virus（NiV） were observed.All the detection results of 101 brain tissue samples of shepherd dogs were negative.Conclusion There is no evidence of HeV infection in the shepherd dogs bred in natural grazing lands in Ili,suggesting low risk of outbreak of HeV infection in the near future.

关 键 词：亨德拉病毒 一步法荧光定量RT-PCR N基因 新疆伊犁地区 牧羊犬 

分 类 号：S852.65[农业科学—基础兽医学]