复发性流产与夫妻双方绒毛血管内皮生长因子基因多态性的关系  

Association of Recurrent Spontaneous Abortion with Parental and Chorionic Villi's Vascular Endothelial Growth Factor Gene Polymorphism in Southern Chinese Population

作　　者：李珠玉[1] 游泽山[1] 苏秀梅[1] 张秀明[2] 李再仪[1] 吴再归[1] 江丽[3] 

机构地区：[1]中山大学附属第一医院妇产科,广东广州510080 [2]中山大学干细胞与组织工程中心研究所,广东广州510080 [3]中山大学附属第一医院骨科研究所,广东广州510080

出　　处：《中山大学学报（医学科学版）》2011年第1期85-89,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2008B030301096);广州市黄埔区科技计划项目(0931)

摘　　要：【目的】探讨复发性自然流产(RSA)与中国南方夫妻双方及绒毛血管内皮生长因子基因多态性(-1154G>A)的关系。【方法】采用等位基因特异性扩增-聚合酶链反应方法,检测271对RSA夫妇和250名女性对照、200名男性对照、60例复发自然流产绒毛及人工流产绒毛的VEGF基因-1154G>A多态位点基因型频率分布情况。【结果】在女方RSA组和对照组,VEGF基因-1154G/A的三种基因型频率(G/G、G/A、A/A)分布不相同,差异有统计学意义(P=0.012);女方RSA组和对照组等位基因频率(G、A)分别是74.7%、25.3%和82.2%、17.8%,女方携带A等位基因发生RSA的风险大于携带G等位基因(P=0.003,OR=1.562,95%CI=1.157-2.109)。在男方RSA组和对照组,VEGF基因-1154G/A的三种基因型频率及等位基因频率分布差异均无统计学意义(P分别为0.837、0.572)。在留取的绒毛RSA组和人工流产组,VEGF基因-1154G/A的三种基因型频率及等位基因频率分布差异均无统计学意义(P分别为0.708、0.757)。【结论】女方VEGF基因-1154G/A多态与中国南方复发性自然流产的发病风险相关,携带A等位基因增加复发性自然流产的发生,男方及绒毛VEGF基因-1154G/A多态与中国南方复发性自然流产的无相关性。由此推测,与胎儿VEGF-1154G/A多态性相比,母方VEGF-1154G/A多态性与RSA的关系可能更密切。【Objective】To explore the association of parental and chorionic villi＇s polymorphisms of vascular endothelial growth factor（VEGF） gene（-1154GA） with recurrent spontaneous abortion（RSA） in southern Chinese population.【Methods】 DNA was extracted from blood samples and chorionic villi,and analyzed by allele-specific PCR.A total of 271 couples with a history of idiopathic RSA,together with 250 age-matched control women and 200 age-matched control men were recruited.The specimens of chorionic villi were collected from 60 cases of spontaneous abortion at early stage and 60 normal early pregnancies.Allele-specific amplification Polymerase chain reaction（ASA-PCR） was applied to analyze the-1154G A genotype.【Results】Statistically significant differences in the GA,AA,and GG genotype frequencies were observed between female patients with RSA and the controls（P=0.012）.The risk of RSA was higher in the carriers of the A allele than in women carrying the G allele（P = 0.003,OR = 1.562,95% CI = 1.157-2.109）.The frequencies of three genotypes of VEGF-1154G A polymorphism had no difference between male patients or chorionic villi with RSA and the controls（P = 0.837 and 0.708）.The allelic frequencies were similar between male patients or chorionic villi with RSA and the controls（P = 0.572 and 0.757）.【Conclusion】VEGF-1154G/A polymorphism of female is associated with susceptibility to RSA;carrying A allele may increase the risk of RSA in southern Chinese women,whereas VEGF-1154G/A polymorphism of male and chorionic villi are not associated with RSA.These results may imply that maternal VEGF-1154G/A polymorphism may be more closely related to RSA than embryo-fetal VEGF-1154G/A polymorphism.

关 键 词：血管内皮生长因子 基因多态性 复发性自然流产 

分 类 号：R714.21[医药卫生—妇产科学]