Gene expression profiling of the whitefly （Bemisia tabaci） Middle East- Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus-infected tobacco  

作　　者：Jun-Min Li Yong-Ming Ruan Fang-Fang Li Shu-Sheng Liu Xiao-Wei Wang 

机构地区：[1]Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou [2]College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, Zhejiang Province, China

出　　处：《Insect Science》2011年第1期11-22,共12页昆虫科学（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China （30730061 ）, the National Basic Research Program of China （2009CB 119203）, andthe earmarked fund for Modem Agro-industry Technology Research System.

摘　　要：Begomoviruses are exclusively transmitted by whitefly （Bemisia tabaci） in a circulative, non-propagative manner. The influences of begomoviruses on whitefly vector are complex with both direct and indirect effects. However, the molecular mechanisms underlying these effects are poorly understood and the transcriptional profiles of whitefly on healthy and virus-infected plants have not yet been studied. Using suppression subtractive hybridization, we investigated the differentially expressed genes in whitefly Middle East - Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus （TYLCCNV） infected tobacco. From the forward cDNA library, 124 differentially expressed expression sequence tags （ESTs） were obtained which represent up-regulated genes in the whiteflies feeding on the infected tobacco. From the reverse library, 112 ESTs were isolated which represent down-regulated genes. Among the up-regulated genes, we identified several genes that are probably involved in direct interaction between whitefly and TYLCCN~, including a 26/29-kDa proteinase that appears to participate in the elimination of foreign proteins, heparan sulfate proteoglycan which mediates the entry of several viruses into host cells and two genes of Rickettsia-a secondary endosymbiotic bacterium of whitefly. In addition, we identified a number of genes involved in metabolism, transcription and translation which might be the result of indirect effects of TYLCCNV on the whitefly via host plants. Collectively, our results suggest that TYLCCNV-infected tobacco changes the gene expression profiles of whitefly via both direct and indirect interactions. This study revealed a number of genes involved in whitefly-TYLCCNV-tobacco interactions and provided useful information for future study on this complex system.Begomoviruses are exclusively transmitted by whitefly （Bemisia tabaci） in a circulative, non-propagative manner. The influences of begomoviruses on whitefly vector are complex with both direct and indirect effects. However, the molecular mechanisms underlying these effects are poorly understood and the transcriptional profiles of whitefly on healthy and virus-infected plants have not yet been studied. Using suppression subtractive hybridization, we investigated the differentially expressed genes in whitefly Middle East - Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus （TYLCCNV） infected tobacco. From the forward cDNA library, 124 differentially expressed expression sequence tags （ESTs） were obtained which represent up-regulated genes in the whiteflies feeding on the infected tobacco. From the reverse library, 112 ESTs were isolated which represent down-regulated genes. Among the up-regulated genes, we identified several genes that are probably involved in direct interaction between whitefly and TYLCCN~, including a 26/29-kDa proteinase that appears to participate in the elimination of foreign proteins, heparan sulfate proteoglycan which mediates the entry of several viruses into host cells and two genes of Rickettsia-a secondary endosymbiotic bacterium of whitefly. In addition, we identified a number of genes involved in metabolism, transcription and translation which might be the result of indirect effects of TYLCCNV on the whitefly via host plants. Collectively, our results suggest that TYLCCNV-infected tobacco changes the gene expression profiles of whitefly via both direct and indirect interactions. This study revealed a number of genes involved in whitefly-TYLCCNV-tobacco interactions and provided useful information for future study on this complex system.

关 键 词：BEGOMOVIRUS Bemisia tabaci suppression subtractive hybridization Tomatoyellow leaf curl China virus WHITEFLY 

分 类 号：S641.2[农业科学—蔬菜学] Q78[农业科学—园艺学]