The presence of six cryptic species of the whitefly Bemisia tabaci complex in China as revealed by crossing experiments  

作　　者：Peng Wang Di-Bing Sun Bao-Li Qiu Shu-Sheng Liu 

机构地区：[1]Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou [2]Department of Entomology, South China Agricultural University, Guangzhou, China

出　　处：《Insect Science》2011年第1期67-77,共11页昆虫科学（英文版）

基　　金：Financial support for this study was provided by the National Basic Research Program of China （2009CB 119203）, the National Natural Science Foundation of China （30730061）, and the Earmarked Fund for Modern Agro-industry Technology Research System of China.

摘　　要：Recent phylogenetic analysis using mitochondrial cytochrome oxidase I （mtCOI） sequences ofBemisia tabaci worldwide indicates that the whitefly comprises at least 24 morphologically indistinguishable but genetically distinct cryptic species. While evidence of reproductive isolation has been reported for some of the putative species, more extensive crossing experiments are required to clarify the systematics of this species com- plex. In this study, we established laboratory cultures for six putative species ofB. tabaci collected in China. We conducted 22 inter-species crosses among the six putative species. The data and those reported previously were collated, and the combined dataset covered all the 30 possible inter-species crosses among the six putative species. Intra-species controls always produced female and male progeny and the proportions of females in the first gen- eration （F1） ranged from 56% to 70%. However, in inter-species crosses female progeny were rarely produced, and the few F1 females produced in four of the 30 inter-species crosses were either sterile or significantly weaker in viability. These results demonstrate a pattern of complete reproductive isolation among the six putative species and show that they are six cryptic species in the B. tabaci complex.Recent phylogenetic analysis using mitochondrial cytochrome oxidase I （mtCOI） sequences ofBemisia tabaci worldwide indicates that the whitefly comprises at least 24 morphologically indistinguishable but genetically distinct cryptic species. While evidence of reproductive isolation has been reported for some of the putative species, more extensive crossing experiments are required to clarify the systematics of this species com- plex. In this study, we established laboratory cultures for six putative species ofB. tabaci collected in China. We conducted 22 inter-species crosses among the six putative species. The data and those reported previously were collated, and the combined dataset covered all the 30 possible inter-species crosses among the six putative species. Intra-species controls always produced female and male progeny and the proportions of females in the first gen- eration （F1） ranged from 56% to 70%. However, in inter-species crosses female progeny were rarely produced, and the few F1 females produced in four of the 30 inter-species crosses were either sterile or significantly weaker in viability. These results demonstrate a pattern of complete reproductive isolation among the six putative species and show that they are six cryptic species in the B. tabaci complex.

关 键 词：Bemisia tabaci biological invasion genetic diversity reproductive incompatibility SYSTEMATICS 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治] Q959.223.1[农业科学—植物保护]