果胶酶水解法对栀子提取液中环烯醚萜类成分的影响  

Intrinsic Viscosity Difference of Gardenia Abstract Treated with Pectinase

作　　者：孟硕[1] 张鹏[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中国实验方剂学杂志》2011年第6期10-12,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金(30772755;30830118);"重大新药创制"科技重大专项(2009ZX09103-317)

摘　　要：目的:研究不同条件下栀子水提液特性黏度在果胶酶处理后的变化。方法:利用乌氏黏度计测量栀子水提液在不同密度、果胶酶用量、温度、时间等条件处理后特性黏度的变化,同时测定了去乙酰基车叶草苷酸甲酯、京尼平-β-D-龙胆双糖苷、栀子苷3个成分的变化。结果与结论:溶液相对密度、加酶量能显著影响栀子水提液酶处理的特性黏度,同时果胶酶也能影响京尼平-β-D-龙胆双糖苷与栀子苷的含量。Objective：This paper describes the intrinsic viscosity difference of Gardenia extract treated with pectinase.Method：The intrinsic viscosity analysis was carried out on an Ubbelohde viscometer to determine the change after treated with different relatively density,amount of pectinase,time,temperature,etc.And the changes of geniposide,genipin-1-β-D-gentiobioside and deacetyl-asperulosidic acid methyl ester were also detremined respectively.Conclusion：Pectinase brought about the change of content of geniposide,genipin-1-β-D-gentiobioside and deacetyl-asperulosidic acid methyl ester,the maximum change rate of them was genipin-1-β-D-gentiobioside.

关 键 词：果胶酶 栀子 特性黏度 

分 类 号：R283.6[医药卫生—中药学]