HPLC同时测定乳癖消颗粒中7种成分的含量  

Simultaneous Determination of Seven Components in Rupixiao Granule by HPLC

作　　者：曹旦华[1] 张剑[1] 朱丹妮[1] 

机构地区：[1]中国药科大学中药复方研究室,南京211198

出　　处：《中国实验方剂学杂志》2011年第6期74-76,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立HPLC同时测定乳癖消颗粒中芍药苷、三七皂苷R1、人参皂苷Rg1、连翘苷、哈巴俄苷、人参皂苷Rb1、去氢木香内酯7种成分的含量。方法:采用AlltimaC18色谱柱(4.6 mm×250 mm,5μm);乙腈-水为流动相,梯度洗脱,检测波长203 nm,柱温30℃,流速1.0 mL·min-1。结果:芍药苷、三七皂苷R1、人参皂苷Rg1、连翘苷、哈巴俄苷、人参皂苷Rb1、去氢木香内酯线性范围分别为3.906 25～125μg(r=0.999),9.375～300μg(r=0.999),37.5～1 200μg(r=1.000),2.187 5～70μg(r=0.999),3.75～120μg(r=0.999),25～800μg(r=0.999),0.625～20μg(r=1.000);平均加样回收率分别为97.95%(RSD 0.71%),99.05%(RSD 1.11%),100.70%(RSD 2.46%),102.74%(RSD 0.35%),103.06%(RSD 0.55%),98.25%(RSD 1.68%),100.16%(RSD 1.82%)。结论:本方法方便、稳定、可靠,可用于乳癖消颗粒的质量控制。Objective：To establish a method for simultaneous determination of seven components such as paeoniflorin,ginsenoside R1,ginsenoside Rg1,forsythin,harpagoside,ginsenoside Rb,dehydrocostuslactone contained in Rupixiao granule.Method：HPLC was conducted on an Alltima C18 column（4.6 mm × 250 mm,5 μm） at 30 ℃;the mobile phase was consisted of acetonitrile（A）-water（B） with gradient elution at the flow rate of 1.0 mL·min^-1 detected at 203 nm.Result：The linear range（mg·L^-1） of paeoniflorin,ginsenoside R1,ginsenoside Rg1,forsythin,harpagoside,ginsenoside Rb,dehydrocostuslactone was 3.906 25-125（r = 0.999）,9.375-300（r = 0.999）,37.5-1 200（r = 1.000）,2.1875-70（r = 0.999）,3.75-120（r = 0.999）,25-800（r = 0.999） and 0.625-20 μg（r = 1.000） respectively.The average recoveries were 97.95%（RSD 0.71%）,99.05%（RSD 1.11%）,100.70%（RSD 2.46%）,102.74%（RSD 0.35%）,103.06%（RSD 0.55%）,98.25%（RSD 1.68%） and 100.16%（RSD 1.82%） respectively.Conclusion：The developed method was convenient,reliable and accurate for the quality control of Rupixiao preparation.

关 键 词：乳癖消颗粒 芍药苷 人参皂苷RG1 哈巴俄苷 连翘苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]