制八角枫HPLC指纹图谱研究  

HPLC Fingerprint Spectrum of Radix Alangii Preparata

作　　者：余启荣[1] 和芳[1] 高柏丽[1] 胡玉霞[1] 

机构地区：[1]北京创立科创医药技术开发有限公司,北京100029

出　　处：《中国实验方剂学杂志》2011年第6期117-120,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技支撑计划项目(2007BAI48B08-5)

摘　　要：目的:建立制八角枫高效液相指纹图谱。方法:采用高效液相色谱法,色谱柱采用Alltech Apollo C18(4.6 mm×250 mm,5μm),以乙腈-缓冲液(缓冲液:0.2 g庚烷磺酸钠、3.5 g磷酸二氢钾定容至1 000 mL)作为流动相梯度洗脱。结果:建立了制八角枫的HPLC指纹图谱,方法学考察符合指纹图谱技术要求。结论:该方法稳定、准确,可靠,为制八角枫质控指标的制定提供了科学依据。Objective：To establish the HPLC fingerprint of the pieces of Radix Alangii Preparata.Method：Using the high performance liquid chromatography,the chromatographic column was Alltech Apollo C18（4.6 mm × 250 mm,5 μm） and acetonitrile-buffer（buffer：0.2 g heptane sulfonate,3.5 g potassium dihydrogen phosphate volume to 1 000 mL） was selected as mobile phase gradient elution were adopeted.Result：Established HPLC fingerprint of R Alangii Preparata,and the results of methodological studymet the technical requirements for fingerprinting.Conclusion：The method is stable,accurate,and reliable,to provide a scientific basis of quality control standard for the R.Alangii Preparata.

关 键 词：制八角枫 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]