藏药中乙酰胆碱酯酶抑制剂的筛选  

Screening of Total Alkaloids from Tibetan Medicine for Acetylcholinesterase Inhibitory Activity

作　　者：杨中铎[1] 任晋[1] 薛鹏辉[1] 杨明俊[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,兰州730050

出　　处：《中国实验方剂学杂志》2011年第6期194-196,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(20802031);兰州理工大学优秀青年教师培养计划项目(Q200904)

摘　　要：目的:从8种藏药中筛选对乙酰胆碱酯酶具有高抑制活性的提取物。方法:药材经乙醇提取、酸碱处理、氯仿和正丁醇萃取后,得到氯仿部分和正丁醇部分。各部分用薄层色谱生物自显影法初步筛选其乙酰胆碱酯酶抑制活性,再用改良的Ellman法进一步测定抑制率及半数抑制浓度(IC50)。结果:8种藏药生物碱提取物的氯仿部分有不同程度的乙酰胆碱酯酶抑制活性,其中止泻木Holarrhena antidysenterica、秦艽花Gentiana straminea、山莨菪Anisodus tanguticus抑制活性最强,IC50分别为6.32,28.20,34.80 mg·L-1。正丁醇部分中,山莨菪的抑制活性显著,IC50为51.70 mg·L-1。结论:止泻木、秦艽花、山莨菪具有显著的乙酰胆碱酯酶抑制活性,说明其含有抑制乙酰胆碱酯酶的成分,为进一步跟踪分离活性成分奠定基础。Objective：To screen acetylcholinesterase inhibitory activity of total alkaloidal extracted from 8 tibetan medicines.Method：Tibetan medicines were extracted by ethanol and treated with hydrochloric acid and base,and then extracted by chloroform and n-butanol to obtain chloroform and n-butanol-soluble fractions.The AChE inhibitory activity was tested by TLC bioautographic method and modified Ellman’s colorimetric method in 96-well microplates.Result：The results indicated that most of the chloroform-soluble fractions among the tested samples exhibited various activities.Holarrhena antidysenterica,Gentiana straminea,Anisodus tanguticus were demonstrated having significant activity with IC50 values of 6.32,28.2 and 34.80 mg·L^-1 respectively.The n-butanol-soluble fractions of A.tanguticus also exhibited AchE inhibitory activity with the IC50value of 51.7 mg·L^-1.Conclusion：H.antidysenterica,G.straminea,A.tanguticus,as sources of AchE inhibitor,are worthy of futher study.

关 键 词：藏药 乙酰胆碱酯酶抑制剂 生物碱 生物自显影法 

分 类 号：R285.5[医药卫生—中药学]