补脾方药对大鼠脾细胞中PKC,Bcl-2和Bax蛋白表达的影响  

Effect of Prescriptions of Reinforcing Spleen on PKC,Bcl-2 and Bax Protein Expression in Spleen in Rats

作　　者：冯晓帆[1] 张立德[1] 

机构地区：[1]辽宁中医药大学,沈阳110032

出　　处：《中国实验方剂学杂志》2011年第6期206-208,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省自然科学基金项目(20102150)

摘　　要：目的:通过观测大鼠脾细胞中蛋白激酶C(PKC),B细胞淋巴瘤/白血病-2基因(Bcl-2)和Bax蛋白表达来探讨补脾方药对脾虚证的作用机制。方法:无特定病原体(SPF)大鼠采用复合方法制成脾虚模型后分别以3种不同配伍补脾方药灌胃3周。采用免疫组化法测定脾细胞中PKC,Bcl-2和Bax蛋白表达。结果:模型组与对照组比较,Bcl-2表达减少而Bax表达增加;中药组与模型组比较,Bcl-2表达增加而Bax表达减少;中药用药组与脾虚模型组比较,PKC表达减少(P<0.05),匀有统计学意义。结论:补脾方药治疗脾虚证的作用机制可能与提高脾细胞中Bcl-2表达,降低Bax和PKC表达有关。Objective：To explore the change of the expression of apoptotic regulatory protein PKC,Bcl-2 and Bax in spleens of spleen deficiency rats.Method：Sixty SPF rats were divided equally into five groups.Expression of Bcl-2 and Bax in spleens was studied by immunohistochemistry method.Result：Compared to the control group,the expression of Bcl-2 was markedly lower in the model group.Compared to the model group,the level of Bcl-2 was significantly increased while levels of Bax and PKC was significantly decreased.Conclusion：Effect mechanism of Spleen-Invigorating recipe on spleen deficiency may be related to the incresed of Bcl-2 as well as the decrese of Bax and PKC.

关 键 词：脾虚 脾 蛋白激酶C B细胞淋巴瘤/白血病-2基因 Bax 

分 类 号：R285.5[医药卫生—中药学]