Sphingobium属细菌土壤中降解异丙隆的特性  

CHARACTERISTICS OF ISOPROTURON DEGRADATION IN SOILS BY STRAINS OF SPHINGOBIUM

作　　者：孙纪全[1] 梁斌[1] 黄星[1] 赵煜坤[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,南京210095

出　　处：《土壤学报》2011年第2期383-388,共6页Acta Pedologica Sinica

基　　金：江苏省社会发展项目(BS2007056);南京科技发展计划项目(200901070)资助

摘　　要：通过在不同环境条件的土壤中接人异丙隆降解菌悬液，研究了Sphingobium属的3株细菌-YBL1、YBL2和YBL3在土壤中降解异丙隆的特性，分析了土壤类型、温度、碳氮源、土壤含水量和菌株接种量等因素对3株细菌降解土壤中异丙隆的影响。结果表明，3株细菌在马肝土（pH6．7）中能够高效降解异丙隆，在红壤（pH4．5）中不能降解异丙隆，菌株YBL3在潮土（pH8．2）中也有较好的降解效果；当接种量低于10。CFU g^-1土时，3株细菌均不能降解马肝土中的异丙隆，接种量高于10^6 CFU g^-1土时，菌株可以高效地降解土壤中的异丙隆；马肝土含水量低于40％时，3株细菌降解土壤中异丙隆的速率与土壤含水量呈正相关关系：在16～37℃范围内，菌株降解马肝土中异丙隆的速率与温度亦呈正相关关系。Through inoculation of isoproturon-degrading bacteria into soils different in environmental conditions, characteristics of the degradation of isoprotaron in the soils by strains Sphingobiurn sp. YBL1, YBL2 and YBL3 were studied, and effects of soil type, temperature, soil moisture, inoculation dosage and carbon/nitrogen source on the degradation were analyzed. It was found that strains YBL1, YBL2 and YBL3 could efficiently degrade isoproturon （ 〉 80% ） in Magan soil （pH 6.7）, but could not in Red soil within 24 hours. The degradation rates of isoproturon in Magan soil by these three strains were positively related to soil moisture （ 10%- 40% ） and temperature （ 16 - 37℃）. When the dosage of in- oculation was less than 105 CFU per gram soil all these three strains were unable to degrade isoproturon in soil in 24 hours after incubation. Supplementation of glucose could enhance degradation of isoproturon in Magan soil by these strains, while the addition of NH4NO3 could do reversely.

关 键 词：异丙隆 微生物降解 土壤 苯脲类除草剂 

分 类 号：S154.39[农业科学—土壤学]