蒿鳖养阴软坚方抗CCl_4复合因素所致大鼠肝纤维化的作用  

Effect of Haobie Yangyin Ruanjian Decoction on rats with hepatic fibrosis induced by CCl_4 composited factor

作　　者：杨凤蕊[1] 娄建石[1] 方步武[1] 

机构地区：[1]天津医科大学药理学教研室,天津300070

出　　处：《中草药》2011年第3期530-534,共5页Chinese Traditional and Herbal Drugs

基　　金：天津市科委应用基础研究计划重点项目(06YFJZJC02900)

摘　　要：目的观察蒿鳖养阴软坚方抗CCl4复合因素致大鼠肝纤维化的作用。方法 Wistar大鼠随机分为对照组,模型组,蒿鳖养阴软坚方低、中、高剂量组,复方鳖甲软肝片及秋水仙碱阳性对照组。CCl4复合因素法建立肝纤维化模型,比色法测定血清丙二醛(MDA)、总蛋白(TP)、白蛋白(ALB)的量及肝组织羟脯氨酸(Hyp)水平,明胶酶谱法检测肝组织基质金属蛋白酶2(MMP-2)及基质金属蛋白酶9(MMP-9)的活性,免疫组化法检测肝组织α-平滑肌肌动蛋白(α-SMA)的表达。结果与模型组相比,随着剂量的增加,蒿鳖养阴软坚方组大鼠血清MDA水平逐渐下降,血清TP、ALB的量逐渐增加,肝组织Hyp的量和MMP-2活性逐渐下降,MMP-9活性变化不明显,肝组织α-SMA的表达下降。结论蒿鳖养阴软坚方能够对抗CCl4复合因素诱发的大鼠肝纤维化。Objective To observe the effect of Haobie Yangyin Ruanjian Decoction（HYRD） on rats with hepatic fibrosis induced by CCl4 composited factor.Methods Wistar rats were randomly divided into seven groups： control group,model group,HYRD groups of low,medium,and high doses,positive groups of Fufang Biejia Rungan Tablets,and colchicin.Model of hepatic fibrosis was induced by CCl4 composited factor.Malondialdehyde（MDA）,total protein（TP）,albumin（ALB） in serum and hydroxyproline（Hyp） in liver were detected by chromatometry;MMP-2 and MMP-9 activities in liver were detected by gelatin zymography;α-SMA expression in liver was evaluated by immunohistochemical method.Results MDA in serum and Hyp in liver were decreased gradually in HYRD groups compared with those of model group;TP and ALB in serum were increased gradually in HYRD groups compared with those of model group;MMP-2 activity in liver was decreased in HYRD groups compared with those of model group;MMP9 activity had no significant change in each group and α-SMA expression was decreased gradually in HYRD groups compared with those of model group.Conclusion HYRD can antagonize the hepatic fibrosis induced by CCl4 composited factor

关 键 词：蒿鳖养阴软坚方(HYRD) CCL4 肝纤维化 羟脯氨酸(Hyp) 基质金属蛋白酶(MMP) 

分 类 号：R285.5[医药卫生—中药学]