阿魏酸钠与苦参素的协同抗炎作用研究  

Observation of synergistic anti-inflammatory effect of combination of sodium ferulate and oxymatrine

作　　者：孙妍[1] 刘汉清[1] 陈方芳[1] 高艳[1] 许明波 刘志峰[1] 

机构地区：[1]烟台大学药学院,山东烟台264005 [2]北京双鹭药业股份有限公司,北京100041

出　　处：《中草药》2011年第3期538-541,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的探讨阿魏酸钠(sodium ferulate,SF)与苦参素(kushenin)即氧化苦参碱(oxymatrine,OMT)联合用药的协同抗炎作用。方法采用小鼠耳肿胀、大鼠足肿胀、大鼠棉球肉芽肿模型观察SF与OMT联合用药的抗炎作用。体外采用RAW264.7细胞观察联合用药对脂多糖(LPS)诱导的NO合成的影响,用Griess法测定NO生成量。结果 SF与OMT联合应用时,可明显降低二甲苯所致的小鼠耳肿胀和角叉菜胶所致的大鼠足肿胀,大剂量可减轻大鼠棉球肉芽肿的质量,但单独给予SF或OMT对上述3种炎症模型均无显著作用。体外实验结果显示,SF与OMT联合应用可明显降低LPS诱导的RAW264.7细胞NO的产生,且二者之间存在协同作用。结论 SF与OMT联合应用具有明显的协同抗炎作用,降低LPS诱导的炎性因子NO的产生,可能是其减轻炎症反应的机制之一。Objective To observe the action of Salvia miltiorrhiza extracts（SmE2,liposoluble constituents） on the formation of physical dependence induced by morphine in mice and the effects of SmE2 on the production of cyclic adenosine monophosphate（cAMP） and nitric oxide（NO） in brain of morphine-dependent mice.Methods Morphine-dependent mice model was established by sc injection of morphine at a gradual increasing dosage.The effect of SmE2 on the withdrawal symptoms induced by Naloxone in morphine-dependent mice was observed.cAMP and NO content in brain were determined through radioimmunoassay and nitrate reductase,respectively.Results SmE2（80 mg/kg） could remarkably reduce the withdrawal symptoms in morphine-dependent mice.The cAMP concentration in brain showed no obvious change in morphine-dependent mice treated with SmE2,but NO content in brain could significantly be reduced.Conclusion SmE2 has effect on relieving the withdrawal symptoms in morphine-dependent mice with down-regulation of NO content in brain as possible mechanism

关 键 词：阿魏酸钠(SF) 苦参素 氧化苦参碱(OMT) 抗炎 协同作用 一氧化氮(NO) 

分 类 号：R285.5[医药卫生—中药学]