流动相离子色谱法同时测定植物中残留的矮壮素和缩节胺  

Simultaneous determination of chlormequat chloride and mepiquat chloride in plants by mobile phase ion chromatography

作　　者：周旭[1,2] 许锦钢[2] 陈智栋[1] 朱岩[2] 

机构地区：[1]常州大学石油化工学院,江苏常州213164 [2]浙江大学化学系,浙江杭州310028

出　　处：《色谱》2011年第3期244-248,共5页Chinese Journal of Chromatography

基　　金：国家自然科学基金(Nos.20775070;J0830413);浙江省自然科学基金(Nos.R4080124;Y4100190)项目

摘　　要：以离子对试剂作流动相,采用离子对抑制电导检测法同时测定植物中残留的矮壮素和缩节胺。简单处理后的样品经过Dionex IonPac NG1保护柱和NS1分离柱,在流速为1.00 mL/min、淋洗液为1.00 mmol/L九氟戊酸(作为离子对试剂)-7%(体积分数,下同)乙腈时等度洗脱分离,能够快速稳定出峰,且被测物与其他干扰离子充分分离。矮壮素和缩节胺的检出限分别为0.154 6和0.171 4 mg/L,在1～100 mg/L范围内具有良好的线性关系和重现性。对实际样品进行测定,矮壮素和缩节胺的回收率范围分别为96.06%～104.6%和98.53%～103.7%,相对标准偏差小于3%。该方法分析结果令人满意,可以满足矮壮素和缩节胺常规的定性、定量分析需求。Ion-pair reagent was used as mobile phase for the simultaneous separation of chlormequat chloride and mepiquat chloride in plants. The rapid separation was performed on a Dionex IonPac NGI guard column and a Dionex IonPac NS1 analytical column using 1. 0 mL/min of the eluent mixture of 1.00 mmol/L nonafluoropentanoic acid （ as ion-pair reagent） and 7% acetonitrile, and the detection was achieved by a suppressed conductivity detector. The method provided good resolution of the analyte peaks without any interference. The detec- tion limits of chlormequat chloride and mepiquat chloride were 0. 154 6 mg/L and 0. 171 4 mg/L, respectively. The linear calibration curves were obtained in the range of 1 - 100 mg/L. The mean recoveries in the ranges of 96.06% - 104.6% for chlormequat chloride and 98.53% - 103.7% for mepiquat chloride were obtained in real samples. The method requires only simple sample preparation and the technique is suitable for routine quality control analysis.

关 键 词：流动相离子色谱 抑制电导检测 矮壮素 缩节胺 植物 

分 类 号：O658[理学—分析化学]