硒化壳聚糖对NB4细胞增殖的影响及其与PML-RARα融合蛋白激活的Ras信号途径的关系  

INHIBITORY EFFECT OF SELENIUM CHISTON ON PROLIFERATION OF NB4 CELLS INVOLVES DOWN-REGULATION OF PML-RARΑ FUSION PROTEIN-INITIATED RAS SIGNAL TRANSDUCTION PATHWAY

作　　者：邓守恒[1] 蔡晓军[1] 佐志刚[1] 张军[1] 雷金华[1] 陈萍[1] 

机构地区：[1]郧阳医学院附属人民医院肿瘤中心,十堰442000

出　　处：《现代预防医学》2011年第6期1074-1076,共3页Modern Preventive Medicine

基　　金：湖北省教育厅基金(B200624004);十堰市科学技术局基金(20061835)

摘　　要：[目的]研究硒化壳聚糖(Sc)对急性早幼粒细胞白血病(APL)细胞株NB4增殖的影响,探讨这种影响与PML-RARα融合蛋白及其激活的Ras信号途径的关系。[方法]NB4为表达PML-RARα融合蛋白阳性的细胞株,K562为表达PML-RARα融合蛋白阴性的细胞株,应用MTT法检测Sc对两种细胞株增殖的影响,应用流式细胞术和Western blot法检测两种细胞株蛋白含量的变化。[结果]Sc对两种细胞株均可产生剂量和时间依赖性的抑制作用,相比之下,对K562细胞的抑制作用明显较弱(P﹤0.05)。Sc处理后,NB4细胞内PML-RARα融合蛋白和c-Jun信号蛋白含量明显减少,而K562细胞c-Jun信号蛋白含量则轻微减少。[结论]Sc可特异性地减少PML-RARα融合蛋白含量从而下调其激活的Ras信号途径,最终抑制NB4细胞增殖。[Ob]ective] To investigate the effects of Selenium ehiston （Sc） on proliferation of NB4 cells and explore the relationship between this effeet and Ras signal transduction pathway activated by PML-RARct fusion proteins. [Methods] NB4 cell line was used as a PML-RARot fusion proteins-positive cell system and K562 cell line as a PML-RARa fusion proteinsnegative control. MTTwas used to determine the proliferate effects of drugs on NB4 and K562 cells. Western blot and flow eytometry were used to examine the abundance of signal protein molecules expressed in tumor cells. [ IqOSults] An exposure of NB4 ce/Js or/（562 ee//s to ,So produced both eoneentration and time-dependent increase in the anti-proliferate rate.Moreover, NB4 cells had more sensitivity to Se than K562 cells （P 〈 0.05）. The abundance of PML-RARa fusion proteins as well as cJUN proteins were strongly down-regulated in Se-treated PML-RARc~ fusion proteins positive NB4 cells while c-JUN proteins were only slightly down-regulated in PML-RARa fimion proteins negative K562 cells. [Gor^lusiorl] Se inhibited the proliferation of NB4 cells and the inhibitory effect was correlated with down-regulation of the abundance of PML-RQRa fusion proteins, which may ultimately lead to retard the Ras signal transductiofi pathway.

关 键 词：硒化壳聚糖 NB4细胞 c.Jun PML-RARet融合蛋白 

分 类 号：R34[医药卫生—基础医学]